Fig 1.
Log2 fold change versus FKPM (Fragments Per Kilobase Million) for each contig.
Significantly DE contigs (FDR < 0.05) are colored in red.
Table 1.
Functional clusters of the DE genes.
Fig 2.
Percentage of contigs with a low, medium, high or very high expression level in female ovaries in our transcriptome data (background, black) compared to the ones found to be DE between diapausing and non-diapausing females (white).
P values are from a one-sided Fisher’s exact test, see text for details: NS = P > 0.05, ***P < 0.001.
Fig 3.
Estimating the effect of the tissue heterogenity.
Normalized gene expressions levels comparing diapausing and non-diapausing D. montana females A) from the RNAseq analysis (for comparison), and B) from the qPCR using the females whose ovaries had been removed before RNA extractions. Both data sets present the combined data for all the three isofemales strains (see text for details). CPM = counts per million. Significance levels for qPCR: •0.1 > P > 0.05, **0.01 > P > 0.001, ***P < 0.001.