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Fig 1.

Coupling the microfluidics and the DVD writer.

(a) Schematic of the microfluidic device on top of the DVD writer. The DVD focus and tracking positions are controlled through an Arduino card, which also controls the on-off switching of the laser. (b-c) Images of the DVD writer, without and with the microfluidic device in place. (d) 3D profilometry of the microfluidic circuit. The colors indicate the local channel depth, as shown on the color bar. The left-most entrance transports the continuous oil phase. The entrance on the right side is used to inject the water phase. The sloped region produces monodisperse drops passively. finally, a default central rail and four side rails are visible on the right side of the image.

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Fig 1 Expand

Fig 2.

Controlling the DVD laser and lens.

The DVD laser was controlled using a constant current source. The power output of the circuit was determined manually by turning the variable resistor. The mobile lens position was controlled through the Arduino motor shield. Its position could be programmed through software. Extreme care must be taken while manipulating the laser, as these are relatively high power lasers that can cause severe eye damage.

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Fig 2 Expand

Fig 3.

Calibration of laser forcing.

(a) Position of the laser spot as a function of the command on the Arduino board, in the quasi-static regime. (b-e) Dynamic traces of the laser position as a function of time filmed through high speed camera. The laser was displaced over a distance of 1.05 mm with a duration of (b) 500 ms, (c) 100 ms, (d) 50 ms, and (e) 20 ms.

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Fig 3 Expand

Fig 4.

Drop size and velocity.

(a) Value of the water inlet pressure that allows a constant droplet size, as a function of the imposed pressure on the oil inlet. (b) Velocity of the droplets, downstream of the sloped region, as a function of the imposed pressure on the oil inlet.

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Fig 4 Expand

Fig 5.

Required power for derailing drops.

Minimum electrical current required to derail droplets onto the first rail. Faster moving droplets require a more intense laser to switch rails. The largest current achieved on this laser was 0.28 A. Drops beyond a velocity of 6 mm/s could not be derailed at this maximum value.

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Fig 5 Expand

Fig 6.

Multiple sorting positions.

Sorting droplets onto different rails by controlling the position of the laser lens. Each panel shows a superposition of images for a single drop in the device. (a) If the laser is off, the drop remains on the default central rail. (b-d) Different laser positions force the drop to jump on different rails, which lead to different regions downstream.

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Fig 6 Expand