Fig 1.
MRI and histopathological findings in a patient with diagnosed cerebral lymphoma.
a. T1w image documenting a lesion with marked homogenous enhancement. b. ADC map. The ADCmin value of the lesion is 64x10-4mm2s-1, the ADCmean value of the lesion is 80×10−4 mm2s-1 and the ADCmax value is 133×10−4 mm2s-1. c. Immunohistochemical stain (MIB-1 monoclonal antibody). Ki 67-index = 50%. d. “Particles tool” analysis image (ImageJ converted MIB-1 image). Cell count (total number of nuclei in the image), total nucleic area (total area of all nuclei in the image) and average nucleic area (total area of all nuclei in the image divided by the number of nuclei in the image) were calculated via the particles tool in ImageJ. For the image shown in fig 1d the following values were determined by the “Particles tool” analysis; cell count = 1424, total nucleic area = 105678.14 μm2 and average nucleic area = 74.37 μm2.
Table 1.
Summary of age, sex and ADC values (ADCmin, ADCmean, ADCmax and deltaADC) of each lesion corresponding to the respective individuum measured as described above.
ADCmin, ADCmean, ADCmax and deltaADCvalues displayed as × 10−4 mm2s-1.
Table 2.
Synopsis of ADC Fractions and histopathological parameters.
Table 3.
Identified correlations between diffusion and histopathological parameters, statistically significant findings (p<0.05) are displayed in bold italics.
Fig 2.
Significant correlations between ADC fractions (displayed as 10−4 mm2s-1) and histopathological parameters (Ki-67: positively stained nuclei displayed in % and total nuclei area: displayed in μm2).
Statistical analysis documenting significant correlations between ADCmin and Ki-67 level (r = -0.454; p = 0.038) (a), ADCmean and Ki-67 level (r = -0.546; p = 0.010) (b), ADCmax and Ki-67 level (r = -0.515; p = 0.017). Significant correlations between ADCmean and total nuclei area (r = -0.500; p = 0.021) (d) are also shown.