Fig 1.
Female flowering of B. papyrifera, Rapanui, 2014.
Tree at left, small branch with female inflorescence at right. The stigmas are exerted but no male tree is nearby for wind pollination. This crater population is isolated and not frequently harvested.
Table 1.
List of samples analyzed.
Fig 2.
Diagram of duplex PCR for sex identification of B. papyrifera.
A. Banding pattern of duplex PCR. Lane1: 1 kb Plus DNA Ladder, Lane 2: Male Amplification Pattern, Lane 3: Female Amplification Pattern. Electrophoresis on 1.5% agarose gel. B. Diagram of amplification products. IAC = Internal Amplification Control, MA = Male Amplification.
Table 2.
List of herbarium accessions with inflorescences.
Fig 3.
Alignment of male and female sequences in the polymorphic region of the B. papyrifera sex marker.
Fig 4.
Banding pattern of a duplex PCR used to identify sex in selected B. papyrifera samples.
Lane 1: 1Kb DNA standard, Lane 2: BQUCH0137 (male control); lane 3: BQUCH0139 (female control); lane 4: BQUCH0194; lane 5: BQUCH0195; lane 6: BQUCH0201; lane 7: BQUCH0202; lane 8: BQUCH0203; lane 9: BQUCH0204; lane 10: BQUCH0204d; lane 11: BQUCH0205; lane 12: BQUCH0208; lane 13: BQUCH0208d; lane 14: BQUCH0209; lane 15: BQUCH0210; lane 16: BQUCH02011; lane 17: BQUCH0212; lane 18: BQUCH013; lane 19: negative PCR control (H2O). Electrophoresis on 1.5% agarose gel.
Fig 5.
Sex distribution of paper mulberry in the sampled native range, Near and Remote Oceania.
Size of circles is proportional to the number of samples (indicated inside each circle).