Fig 1.
Colonial characteristic and microscopic morphology of C. bantiana.
The (A) surface and (B) close-up view of the colonial morphology of C. bantiana after being cultured for seven days. Light micrograph showing (C) smooth walled, pale olivaceous, ellipsoidal to spindle-shaped conidia arranged in long, strongly coherent chains (400× magnification, bars 20 μm).
Fig 2.
Bayesian phylogram generated using the combined gene sequences of ITS and LSU.
The tree was rooted with Plococarpus schaereri AFTOL-ID 2289 as outgroup. The numbers on the nodes indicate Bayesian posterior probability based on 100 sampling frequencies for a total of 100,000 generations.
Table 1.
Details of Isolates Subjected to Multilocus Phylogenetic Analysis.
Table 2.
C. bantiana UM 956 genomic and assembly features.
Table 3.
Genome content of C. bantiana UM 956 and other previously sequenced fungal genomes.
Fig 3.
KOG class annotation distribution of C. bantiana UM 956 genome.
A total of 7,185 of protein-coding genes were annotated by KOG. The proteins were assigned into different KOG functionary categories as shown in the pie chart.
Table 4.
Putative transposable elements in the genome sequence of C. bantiana UM 956.
Fig 4.
KEGG classifications of proteins in C. bantiana UM 956 genome.
The proteins were assigned into different KEGG metabolic pathway categories as shown in the pie chart. A total of 2,506 protein-coding genes were involved in metabolic pathway based on the KEGG database.
Fig 5.
CAZyme class annotation distribution of C. bantiana UM 956 genome.
(A) Comparison of the distribution of CAZyme catalytic domains between C. bantiana and fungi from various lifestyles. (B) Comparison of the plant cell wall degrading potential from CAZyme analysis between C. bantiana and fungi from various lifestyles. AA: auxiliary activities; CBM: carbohydrate binding module; CE: carbohydrate esterase; GH: glycoside hydrolase; GT: glycosyltransferase and PL, polysaccharide lyase.
Fig 6.
(A) Three-dimensional ribbon structures of CbSAP3 and C. albicans SAP3. α-helices are shown in red; β-sheets are shown in yellow and random coils are shown in black. The N- and C- terminal end are labeled. (B) Active sites comparison of the CbSAP3 and C. albicans SAP3. Carbon atoms are shown in green; nitrogen atoms are shown in blue; oxygen atoms are shown in red. Hydrogen bonds are shown in pink dotted lines.
Fig 7.
Siderophore genes of C. bantiana UM 956 genome.
(A) FC synthetase SidC gene. (Top panel) Schematic map of SidC (black arrow) and adjacent genes. Gray arrows are genes which are expected to function during siderophore biosynthesis. Numbers are in kilobases. (Bottom panel) Domain setup of SidC. (B) FSC synthetase SidD gene. (Top panel) Schematic map of SidD (black arrow) and adjacent genes. Numbers are in kilobases. (Bottom panel) Domain setup of SidD.
Fig 8.
Comparative phylogenomic analysis of C. bantiana UM 956 along with eight previously published dematiaceous fungal genomes using Bayesian.
Number at the node referring to Bayesian posterior probability. The tree is rooted with C. neoformans var. grubii H99 as outgroup.
Table 5.
Shared gene families in both C. bantiana UM 956 and E. dermatitidis NIH/UT8656.
Table 6.
Specific functional family clusters in C. bantiana UM 956.