Fig 1.
THBS2 was overexpressed in lung adenocarcinoma tissue.
(A) Expressions of THBS1 and THBS2 in 20 common cancers were compared with corresponding normal tissues (Oncomine Database). The threshold of search criteria for datasets of cancer vs. normal analysis was a p-value <1E-4, a fold change >2, and a gene rank in top 5%. Analysis of (B) THBS1 and (C) THBS2 mRNA expression in lung normal tissue and lung AC tissue by using Oncomine database. Relative (D) THBS1 and (E) THBS2 expression of six human lung AC cell lines (A549, H1299, CL1-0, CL1-1, CL1-2 and CL1-5) compared with the mean value of a normal lung cell line (IMR90). THBS1, Thrombospondin 1. THBS2, Thrombospondin 2. AC, adenocarcinoma.* represent a P value < 0.05.
Table 1.
mRNA expression levels of THBS1 and THBS2 in lung adenocarcinoma.
Fig 2.
Expression level of THBS2 was associated to prognosis in patients with lung adenocarcinoma.
(A)Kaplan-Meier survival curve (Kaplan-Meier plotter) showing the progression-free survival of THBS1 and THBS2 in lung adenocarcinoma. (B) Survival of lung AC patients who never smoked with THBS1 and THBS2 expression. (C) Survival of lung AC patients who were smokers with THBS1 and THBS2 expression. High and low expression in patients was represented in red and black, respectively. (D) Kaplan-Meier survival curve (SurvExpress, Okayama Lung dataset) showing the survival of lung AC patients with high or low THBS2 expression. High and low expressions were represented in red and green, respectively. (E) THBS2 expression levels with low and high risk groups generated from Okayama Lung dataset by using SurvExpress program. THBS, Thrombospondin. AC, adenocarcinoma.
Table 2.
Analysis of THBS1 and THBS2 in lung cancer.
Fig 3.
Prognosis analysis of THBS2 coexpressed genes in lung adenocarcinoma.
(A) THBS2 coexpressed gene pattern in lung adenocarcinoma (Landi lung dataset). (B) 9 genes (CHI3L1, COL11A1, COL5A2, CYP1B1, FAP, MXRA5, THY1, VCAM1 and VCAN) were consistently identified as top 5% genes by co-expression score with Oncomine in lung AC datasets. (C) Kaplan-Meier survival curve (Kaplan-Meier plotter) showing the association of CHI3L1, COL11A1, COL5A2, FAP, MXRA5, THY1 and VCAN with progression-free survival in lung AC. (D) Kaplan-Meier survival curve showing the progression-free survival in lung AC with both high THBS2 and CYP1B1 expression, or both low THBS2 and CYP1B1 expression. AC, adenocarcinoma.
Table 3.
Analysis of genes co-expressing with THBS2 appeared in three lung adenocarcinoma datasets.
Fig 4.
Prognosis analysis of THBS1 coexpressed genes in lung adenocarcinoma.
(A) THBS1 coexpressed gene pattern in lung adenocarcinoma (Landi lung dataset). (B) 13 genes (CCL5, CD3D, CDH11, CDKN1A, CORO1A, FYB, GBP1, GZMK, HLA-DQA1, LOXL1, OLFML2B, PDE4DIP, and SELL) were consistently identified as top 5% genes by co-expression score with Oncomine in lung AC datasets. (C) Kaplan-Meier survival curve (Kaplan-Meier plotter) showing the association ofCCL5, CDH11, FYB, GZMK, HLA-DQA1, PE4DIP, and SELL with progression-free survival of in lung AC. AC, adenocarcinoma.
Table 4.
Analysis of genes co-expressing with THBS1 appeared in three lung adenocarcinoma datasets.
Fig 5.
Down-regulation of VCAN or THBS2 decreased the cell proliferation in A549 cells.
(A) mRNA expression level of VCAN was determined with real-time PCR. (B) mRNA expression level of THBS2 was determined by using real-time PCR. (C) The proliferation of A549 cells with VCAN or THBS2 knockdown expression were examined by WST-1 assay.* represent a P value < 0.05. ** represent a P value < 0.005.
Fig 6.
Gene ontology analysis and network construction of THBS2 coexpressed genes.
(A) Gene ontology analysis of THBS2 coexpressed genes were conducted by using the DAVID (Database for Annotation, Visualization and Integrated Discovery) bioinformatics tool. P<0.001 for the pathway enrichment of THBS2 coexpressed genes compared with Homo sapiens transcriptome background. (B) The biological interactions of THBS2 coexpressed genes were analyzed by using Metacore. Red line, negative/ inhibition effects; Green line, positive/ activation effects. Gray line, unspecified effects.