Fig 1.
Rhesus monkey Tas1r2 responds to natural sugars, amino acids, artificial sweeteners and sweet-tasting proteins but not sweet taste inhibitor amiloride.
(A) The responses of rhTas1r2/hTAS1R3 towards HBSSH (buffer solution), sucrose (300 mM), glucose (300 mM), trehalose (250 mM), D-tryptophan (5 mM), sucralose (1 mM), perillartine (300 μM), stevioside (1 mM), D-glucosamine (500 mM), monellin (45 μM), thaumatin (4.4 μM) and curculin (10 μM). (B) The responses towards HBSSH, aspartame (5 mM), neotame (0.25 mM), saccharin (1 mM) and amiloride (3 mM). The asterisks indicate significant levels of the receptor responses to various sweeteners determined by the two-sided unpaired t test (*: p<0.05; **: p<0.01; ***: p<0.001) compared with the receptor responses to HBSSH buffer (S1 and S2 Appendices).
Fig 2.
Perillartine activates the Tas1r2 subunit in a species-dependent manner.
(A) Responses of the Tas1r2 subunits of human, rhesus monkey, squirrel monkey and mouse, rhTas1r2/mTas1r3 and mTas1r2/mTas1r3 towards HBSSH (buffer solution), perillartine (300 μM) and cyclamate (50 mM). The asterisks indicate significant levels of the receptor responses to the sweeteners determined by the two-sided unpaired t test (*: p<0.05; **: p<0.01; ***: p<0.001) compared with the receptor responses to HBSSH buffer (S3 Appendix). (B) Dose-response curve of the Tas1r2 subunits of human, rhesus monkey, squirrel monkey and mouse towards perillartine.
Fig 3.
Homology modeling of the full length rhesus monkey Tas1r2.
The conserved VFTM, CRD and TMD are colored in red, aqua and blue, respectively.
Fig 4.
Sequence alignment of rhesus monkey Tas1r2 with the functionally characterized Tas1r2s from other species.
Conserved residues are indicated by asterisk above the alignment, single and double dots represent amino acids with semi-conservative and conservative characteristics. Gaps introduced during the alignment process are indicated as dashes. The conserved VFTM, CRD and TMD regions are underlined, boxed and grey color shaded respectively. The conserved aspartame and D-tryptophan interactive residues Y103, S144, E302 and D307 (numbering based on the rhTas1r2 sequence) are denoted as black triangles, and residues T40, D142 and D278 responsible for the species-dependent taste to aspartame are denoted as white triangles. Saccharin-specific residues E382 and R383 are denoted as black cycles, and residues S165 and P277 specific for D-tryptophan or sucralose reception are denoted as white cycles [3, 12, 15]. rhTas1r2: rhesus monkey Tas1r2 (GenBank: ABD37678); hTAS1R2: human TAS1R2 (Q8TE23); smTas1r2: squirrel monkey Tas1r2 (A3QP08); gpTas1r2: giant panda Tas1r2 (XP_002926877); mTas1r2: mouse Tas1r2 (Q925I4); rTas1r2: rat Tas1r2 (Q9Z0R7).