Table 1.
Primers used in RT-PCR.
Fig 1.
Short-term NaCl rinsing enhanced wound healing in hGFs.
A) Scratch-test assay. B) Remaining wound area was normalized with the time point 0 h. C) NaCl rinsing did not alter the proliferation of hGFs by MTT assay. One-way ANOVA vs. 0% NaCl, *p< 0.05, **p<0.01, ***p<0.001, scale bar 100 μm.
Fig 2.
Short-term NaCl rinsing enhanced the migration of hGFs.
A) Migrated cells were stained with crystal violet after transwell migration assay. Migrated cells B) and cell area C) were measured and compared by one-way ANOVA vs. 0% NaCl, scale bar 100 μm.
Fig 3.
Short-term NaCl rinsing did not affect hNOKs.
A) Scratch-test assay. B) Cell proliferation by MTT assay. C) Cell migration by transwell migration assay. One-way ANOVA vs. 0% NaCl, n = 3, scale bar 100 μm.
Fig 4.
Short-term treatment at 1.8% NaCl altered the expression of COL1, Fn and FAK.
A) mRNA expression by RT-PCR. B) Band-density quantitative analysis. Independent t-test 0% vs. 1.8% NaCl.
Fig 5.
Short-term treatment at 1.8% promoted the expression of FAK and F-actin in hGFs by immunofluorescence, (head arrows: peripheral FAK scale bar 50 μm).
Fig 6.
Chloride involved in the migration of hGFs.
A) Scratch-test assay. B) Remaining wound area was normalized with the time point 0 h. C) NaCl, KCl rinsing did not alter the proliferation of hGFs by MTT assay while NaH2PO4, KH2PO4 did. D) NaCl, KCl rinsing increased migrated cells by transwell migration assay. One-way ANOVA vs. 0% NaCl, scale bar 100 μm. E) Gene expression of COL1, Fn, FAK with and without NaCl, KCl, NaH2PO4 and KH2PO4 rinsing by RT-PCR.