Fig 1.
In vitro biofilm formation on denture materials in human saliva.
Pre-sterilized denture material was inoculated with 1 x 106 C. albicans DAY185 (A, F, K), efg1-/- (B, G, L), efg1-reconstituted (C, H, M), bcr1-/- (D, I, N) or bcr1-reconstituted (E, J, O) strain and incubated in saliva for 24h at 37°C to allow biofilm growth. The denture materials were then processed for SEM (A-E) or stained with calcofluor white (blue; stains fungal chitin in the cell wall) or Concanavalin A-Texas Red conjugate (red; stains mannose in the cell wall and ECM) and examined by fluorescent confocal microscopy to visualize biofilms in XY (F-J) and XYZ (K-O) views. Each panel shows a representative image of 3 repeats. Scale bar = 50 μm.
Fig 2.
Ex vivo biofilm formation on rat palate tissue.
Excised rat palate tissues were inoculated with 1 x 106 C. albicans DAY185 (A, F, K), efg1-/- (B, G, L), efg1-reconstituted (C, H, M), bcr1-/- (D, I, N) or bcr1-reconstituted (E, J, O) strain and incubated in PBS for 24h at 37°C to allow biofilm growth. The tissues were then processed for SEM (A-E) or stained with calcofluor white (blue; stains fungal chitin in the cell wall) or Concanavalin A-Texas Red conjugate (red; stains mannose in the cell wall and ECM) and examined by fluorescent confocal microscopy to visualize biofilms in XY (F-J) and XYZ (K-O) views. Each panel shows a representative image of 2 repeats. Scale bar = 50 μm.
Fig 3.
The role of C. albicans EFG1 and BCR1 in biofilm formation on dentures in vivo.
Equilibrated rats (n = 4/group) were weaned onto gel diet and fitted with dentures. Rats were given broad-spectrum antibiotics in the drinking water for 4 days prior to inoculation. Rats were inoculated 3x at 3-day intervals with 1x109 CFU C. albicans DAY185 (A, F, K), efg1-/- (B, G, L), efg1-reconstituted (C, H, M), bcr1-/- (D, I, N) or bcr1-reconstituted (E, J, O) strain. Dentures were removed from inoculated rats at 4 weeks post-inoculation. The dentures were processed for SEM (A-E) or stained with calcofluor white (blue; stains fungal chitin in the cell wall) or Concanavalin A-Texas Red conjugate (red; stains mannose in the cell wall and ECM) and examined by fluorescent confocal microscopy to visualize biofilms in XY (F-J) and XYZ (K-O) views. Each panel shows a representative image of 2–3 animals. Scale bar = 50 μm.
Fig 4.
The role of C. albicans EFG1 and BCR1 in biofilm formation on palate tissue in vivo.
Equilibrated rats (n = 4/group) were weaned onto gel diet and fitted with dentures. Rats were given broad-spectrum antibiotics in the drinking water for 4 days prior to inoculation. Rats were inoculated 3x at 3-day intervals with 1x109 CFU C. albicans DAY185 (A, F, K), efg1-/- (B, G, L), efg1-reconstituted (C, H, M), bcr1-/- (D, I, N) or bcr1-reconstituted (E, J, O) strain. Palate tissues were excised from inoculated rats at 4 weeks post-inoculation. The tissue samples were then processed for SEM (A-E) or stained with calcofluor white (blue; C. albicans) or Concanavalin A-Texas Red conjugate (red; ECM) and examined by fluorescent confocal microscopy to visualize biofilms in XY (F-J) and XYZ (K-O) views. Each panel shows a representative image of 2–3 animals. Scale bar = 50 μm.
Fig 5.
Quantification of biofilm thickness on dentures and palate tissue in vivo.
Equilibrated rats were weaned onto gel diet and fitted with dentures. Rats were given broad-spectrum antibiotics in the drinking water for 4 days prior to inoculation. Rats were inoculated 3x at 3-day intervals with 1 x 109 C. albicans DAY185, efg1-/- or bcr1-/- strain. (A) Dentures and (B) palate tissues were removed from inoculated rats at 4 weeks post-inoculation. Samples were stained with calcofluor white (blue; stains fungal chitin in the cell wall) or Concanavalin A-Texas Red conjugate (red; stains mannose in the cell wall and ECM) and examined by fluorescent confocal microscopy to visualize biofilms. Cross-sectional images of biofilms were visualized by confocal microscopy at 600X magnification, and the depths of biofilms were measured using the Fluoview software. Figure represents cumulative results from 2 independent experiments with 2–3 animals per group and assessment of 5 random areas per animal. Data were analyzed using a one-way ANOVA followed by the Tukey’s post hoc multiple comparison test. *, P < 0.05; **, P < 0.01 compared to the WT control.
Fig 6.
Fungal colonization levels on dentures and palate tissue in rats inoculated with C. albicans biofilm deficient strains.
Equilibrated rats were weaned onto gel diet and fitted with dentures. Rats were given broad-spectrum antibiotics in the drinking water for 4 days prior to inoculation. Rats were inoculated 3x at 3-day intervals with 1 x 109 C. albicans DAY185, efg1-/- or bcr1-/- strain. Swab samples of both the palate (A) and denture (B) were taken weekly for a period of 8 weeks post-inoculation. Fungal burdens were assessed from swab suspension fluid. Figure represents cumulative results from 2 independent experiments with 4–5 animals per group. Data were analyzed using Repeated Measures ANOVA (longitudinal data for each group) and the unpaired Student’s t test (individual time points (weeks), experimental vs. control). *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Fig 7.
Mucosal damage in rats fitted with dentures inoculated with C. albicans biofilm deficient strains.
Equilibrated rats were fitted with dentures, weaned onto gel diet, given antibiotics in the drinking water, and inoculated 3 times at 3 day intervals with 1 x 109 C. albicans DAY185, efg1-/- or bcr1-/- or reconstituted mutants. Swab samples of the palate were taken weekly for a period of 4 weeks post-inoculation. LDH levels were assessed from swab suspension fluid. The results represent cumulative data from 2 independent experiments at 1–4 weeks post-inoculation with 4–5 animals per group. Data were analyzed using the Kruskal-Wallis test followed by the post hoc Mann-Whitney U test. n.s., not significant; **, P < 0.01; ***, P < 0.001.
Fig 8.
Body weight change over time in rats fitted with dentures inoculated with C. albicans biofilm deficient strains.
Equilibrated rats were fitted with dentures, weaned onto gel diet, given antibiotics in the drinking water, and inoculated 3x with 1 x 109 C. albicans DAY185, efg1-/- or bcr1-/-. Rats were weighed bi-weekly for a period of 8 weeks post-inoculation using 3–4 animals per group and data are shown as (A) absolute weights and (B) % weight change (% weight change = weight at time point/weight at week 0 prior to inoculation). Data for individual weight changes per group were analyzed using the Repeated Measures ANOVA. Weight changes between groups at specific time points (experimental vs. control, actual weight or % change) were analyzed by the unpaired Student’s t test. * P < 0.05.