Fig 1.
Discovery of six special regions in Y chromosome.
A and B show the six highly covered regions (highlighted by red line) in Y chromosome in female and male group. They were located at the same positions of Y chromosome in two groups. C) There are detection counts of six regions in Y chromosome of 92 samples. D) The comparison of Y chromosome reads percentage in the female and male fetus groups in before and after filtering out the reads in six regions of Y chromosome.
Table 1.
Basic information of 92 samples of pregnant women.
Table 2.
The value for calculation of fetal DNA concentration.
Table 3.
The calculated percentage of Y chromosome using original and optimized methods.
Table 4.
The six regions discovered in Y chromosome.
Fig 2.
Testing of the optimized method in large-scale sample.
A) The DNA percentages of Y chromosome calculated by optimized method and original method. Here, the fetal DNA concentration is assumed as 1%. The percentage of Y chromosome calculated by the optimized method was marked as green point. The blue point illustrates the percentage of Y chromosome calculated by original method. B and C) the percentage of Y chromosome calculated by optimized method and original method in 1590 samples. Here, black and red dots represent the percentage of Y chromosome in female fetuses and male fetus, respectively. Error prediction results were marked by blue and green dots in Fig C in which two male fetuses were predicted to be female and marked by blue; a female fetus predicted to be male and marked by green.