Fig 1.
Sperm cells captured by anti-PH-20 IMB.
(A) Optical microscopy image for IMB bound to the head of intact sperm cells. (B) Optical microscopy image for IMB bound to the sperm without midpiece and tail. (C) Scanning electron microscopy image for IMB bound to the head of an intact sperm cell. Scale bars are indicated in each panel.
Fig 2.
Treatment with DNase I yields a single-sourced DNA profile.
(A–C) Electrophoretogram of (A) female epithelial cells, (B) sperm and (C) the IMB–sperm complexes before treatment with DNase I with red circles indicating female DNA contamination. Female epithelial cell and sperm concentrations were both 105/mL. (D) Single-sourced DNA profile of the IMB–sperm complexes after treatment with DNase I.
Table 1.
Comparison of the sensitivity of different antibodies in capturing sperm.
Table 2.
Comparison between anti-PH-20 IMB and differential lysis.