Table 1.
Materials prepared with reagents of different molar ratios.
Fig 1.
Structure of the amphiphilic comb-shaped copolymer PEP and biodegradation properties of PEP nanoparticles.
(a) 1H-NMR spectrum of PEP in DMSO-d6; (b) 1H-NMR spectrum of PEP NPs in D2O; (c) chemical composition percentages of azote on the surface of different NPs; (d) Biodegradation properties of PEP and PLA NPs in PBS (0.1 M, pH 7.4) at 37°C.
Fig 2.
TEM image of PEP NPs.
Table 2.
WU, LC, zeta potential, and particle size of NPs.
Fig 3.
Protein release profiles and MTT assay of PEP NPs in the HL-7702 cell line.
Protein release profiles of (a) PLA NPs, (b) PEP NPs, Cell viability result of (c) PLA NPs, (d) EPL, (e) PEP NPs. Cell viability was determined via MTT assay and expressed as a percentage of the control (100% of longitudinal coordinate).
Table 3.
Kinetic fitting results of BSA released from nanoparticles with different kinetic models.
Table 4.
Acute toxicity of PEP52 NPs, PLA NPs, and EPL.
Fig 4.
H&E-stained section of the liver, spleen, and kidney after injection of PEP52 NPs in vivo.
Fig 5.
Microclimate pH of NPs and circular dichroism spectra of BSA.
(a) Microclimate pH inside PLA and PEP52 NPs over 32 days. After the 16th day, the microclimate pH inside the PLA5 NPs was less than 5.8; (b) circular dichroism spectra of BSA released from PEP NPs and (c) PLA NPs over 32 days in PBS (0.1 M, pH 7.4).
Fig 6.
Time course of BSA-FITC concentration in plasma (a) and tissues [liver (b), spleen (c), and kidney (d)] over 16 days after intravenous injection of free BSA-FITC solution and different BSA-FITC-loaded carriers.
Table 5.
AUC values of BSA-FITC-loaded formations and free BSA-FITC in the tissues and blood of Kunming mice (n = 10).