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Fig 1.

Conceptual framework that underlies the Cochrane risk of bias tool for RCTs.

Letters A-E denote the sources of bias listed in Table 1.

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Table 1.

Eligible sources of bias in randomized trials.

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Fig 2.

Flow diagram of identification, screening, and inclusion of trials.

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Table 2.

Summary of characteristics of included meta-epidemiological studies.

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Table 3.

Heterogeneity associated with methodological characteristics.

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Fig 3.

Random-effects meta-analysis of RORs associated with inadequate/unclear (versus adequate) sequence generation.

The boxed section displays the average bias estimates, where available, from the seven meta-epidemiological studies contributing to the BRANDO 2012a study (however only the BRANDO 2012a ROR was included in our meta-analysis). The BRANDO 2012a ROR is based on a multivariable analysis with adjustment for allocation concealment and double blinding [the corresponding univariable ROR is (95% CrI) 0.89 (0.82, 0.96)]. The BRANDO 2012b ROR is based on a multivariable analysis with adjustment for allocation concealment and double blinding [the corresponding univariable ROR (95% CrI) is 0.89 (0.75, 1.05)]. The Unverzagt 2013c ROR is based on a multivariable analysis with adjustment for allocation concealment, double blinding, attrition, selective outcome reporting, early stopping, pre-intervention, competing interests, baseline imbalance, switching interventions, sufficient follow-up, and single- versus multi-centre status [the corresponding univariable ROR (95% CI) is 0.98 (0.8, 1.21)]. The BRANDO 2012d ROR is based on a multivariable analysis with adjustment for allocation concealment and double blinding [the corresponding univariable ROR (95% CrI) is 0.99 (0.84, 1.16)]. The BRANDO 2012e ROR is based on a multivariable analysis with adjustment for allocation concealment and double blinding [the corresponding univariable ROR (95% CrI) is 0.83 (0.74, 0.94)].

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Fig 4.

Random-effects meta-analysis of RORs associated with inadequate/unclear (versus adequate) allocation concealment.

The boxed section displays the average bias estimates, where available, from the seven meta-epidemiological studies contributing to the BRANDO 2012a study (however only the BRANDO 2012a ROR was included in our meta-analysis). The BRANDO 2012a ROR is based on a multivariable analysis with adjustment for sequence generation and double blinding [the corresponding univariable ROR (95% CrI) is 0.93 (0.87, 0.99)]. The BRANDO 2012b ROR is based on a multivariable analysis with adjustment for sequence generation and double blinding [the corresponding univariable ROR (95% CrI) is 0.98 (0.88, 1.10)]. The BRANDO 2012c ROR is based on a multivariable analysis with adjustment for sequence generation and double blinding [the corresponding univariable ROR (95% CrI) is 0.97 (0.85, 1.10)]. The BRANDO 2012d ROR is based on a multivariable analysis with adjustment for sequence generation and double blinding [the corresponding univariable ROR (95% CrI) is 0.85 (0.75, 0.95)].

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Fig 5.

Random-effects meta-analysis of RORs and dSMDs associated with presence (versus absence) of baseline imbalance.

The Unverzagt 2013a ROR is based on a multivariable analysis with adjustment for sequence generation, allocation concealment, double blinding, attrition, selective outcome reporting, early stopping, pre-intervention, competing interests, switching interventions, sufficient follow-up, and single- versus multi-centre status [the corresponding univariable ROR (95% CI) is 0.92 (0.80, 1.06)].

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Fig 6.

Random-effects meta-analysis of RORs and dSMDs associated with lack of/unclear blinding of participants (versus blinding of participants).

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Fig 7.

Random-effects meta-analysis of RORs and dSMDs associated with lack of/unclear blinding of personnel or participants/personnel (versus blinding of either party).

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Fig 8.

Estimated RORs and dSMDs associated with any (versus no or minimal) attrition.

The boxed section displays the average bias estimates, where available, from the four meta-epidemiological studies contributing to the BRANDO 2012 study. The Abraha 2015a ROR is based on a multivariable analysis with adjustment for use of placebo comparison, sample size, type of centre, items of risk of bias, post-randomisation exclusions, funding, and publication bias [the corresponding univariable ROR (95% CI) is 0.83 (0.71, 0.97)]. The Unverzagt 2013b ROR is based on a multivariable analysis with adjustment for sequence generation, allocation concealment, double blinding, selective outcome reporting, early stopping, pre-intervention, competing interests, baseline imbalance, switching interventions, sufficient follow-up, and single- versus multi-centre status [the corresponding univariable ROR (95% CI) is 1.19 (0.98, 1.45)]. The Nuesch 2009bc dSMD is based on a multivariable analysis with adjustment for allocation concealment [the corresponding multivariable dSMD (95% CI) with adjustment for blinding of participants is -0.15 (-0.30, 0.00), and the corresponding univariable dSMD (95% CI) is -0.13 (-0.29, 0.04)].

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Fig 9.

Random-effects meta-analysis of RORs and dSMDs associated with lack of/unclear blinding of outcome assessors (versus blinding of outcome assessors).

RHR = Ratio of hazard ratios. Hróbjartsson 2014aa “standard trials” comprise those comparing experimental interventions with standard control interventions, such as placebo, no-treatment, usual care or active control. Hróbjartsson 2014ab “atypical trials” comprise those comparing an oral experimental administration of a drug with the intravenous control administration of the same drug for cytomegalovirus retinitis.

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Fig 10.

Random-effects meta-analysis of RORs associated with lack of/unclear double blinding (versus double blinding).

The boxed section displays the average bias estimates, where available, from the seven meta-epidemiological studies contributing to the BRANDO 2012a study (however only the BRANDO 2012a ROR was included in our meta-analysis). The BRANDO 2012a ROR is based on a multivariable analysis with adjustment for sequence generation and allocation concealment [the corresponding univariable ROR (95% CrI) is 0.87 (0.79, 0.96)]. The BRANDO 2012b ROR is based on a multivariable analysis with adjustment for sequence generation and allocation concealment [the corresponding univariable ROR (95% CrI) is 0.92 (0.80, 1.04)]. The Unverzagt 2013c ROR is based on a multivariable analysis with adjustment for sequence generation, allocation concealment, attrition, selective outcome reporting, early stopping, pre-intervention, competing interests, baseline imbalance, switching interventions, sufficient follow-up, and single- versus multi-centre status [the corresponding univariable ROR (95% CI) is 0.84 (0.69, 1.02)]. The BRANDO 2012d ROR is based on a multivariable analysis with adjustment for sequence generation and allocation concealment [the corresponding univariable ROR (95% CrI) is 0.93 (0.74, 1.18)]. The BRANDO 2012e ROR is based on a multivariable analysis with adjustment for sequence generation and allocation concealment [the corresponding univariable ROR (95% CrI) is 0.78 (0.65, 0.92)].

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Fig 11.

Random-effects meta-analysis of RORs and dSMDs associated with high/unclear (versus low) risk of bias due to selective reporting.

The Unverzagt 2013a ROR is based on a multivariable analysis with adjustment for sequence generation, allocation concealment, double blinding, attrition, early stopping, pre-intervention, competing interests, baseline imbalance, switching interventions, sufficient follow-up, and single- versus multi-centre status [the corresponding univariable ROR (95% CI) is 0.73 (0.54, 0.98)].

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