Fig 1.
A) Fluorescence-intensity images of three hN2 cells at 12 h observed in the blue channel (460–480). GP scale to pseudo color the intensity image is shown at the right. C) GP histogram from the corresponding image (membrane) in B). One Gaussian component is observed referring to the cell membrane after digital mask application. Average GP = 0.062.The width at half maximum is ~ 0.1.
Fig 2.
GP distribution in a) hN2, b) HEK293, c) NIH3T3 and d) L6. a) Histograms of GP distributions among hN2 cells at 12 h, 48 h, 72 h and 92 h respectively. At 72 h GP reached minimum values (0.17) ± SEM and increased to 0.28 ± SEM after 92 h. 10 cells were analyzed for each time point for statistical significance. b) Histograms of GP distributions among 10 HEK293 cells at 12 h, 48 h, 72 h and 92 h respectively. At 72 h GP values reached minimum values (0.2) ± SEM and increased to 0.327 ± SEM after 92 h. 10 cells were analyzed for each time point for statistical significance. c) Histograms of GP distributions among 10 NIH3T3 cells at 12 h, 48 h, 72 h and 92 h respectively. At 72 h GP reached maximum values (0.45) ± SEM and decreased to 0.25 ± SEM after 92 h. 10 cells were analyzed for each time point for statistical significance. d) Histograms of GP distributions among L6 cells at 12 h, 48 h, 72 h and 92 h respectively. At 72 h GP reached minimum values (0.17) ± SEM and increased to 0.22 ± SEM after 92 h. 10 cells were analyzed for each time point for statistical significance.
Fig 3.
GP measurements in hN2, HEK293, NIH3T3 and L6 cells at a) 12 h; b) 72 h and c) 92 h.