Fig 1.
(A) yellow flower line. (B) white flower line.
Fig 2.
Phylogenic tree of UGTs from C.tinctorius.
The phylogenetic relationship of UGTs from C.tinctorius and other plants were analyzed with ClustalX and MEGA5 using the amino acid sequences.
Fig 3.
A multiple alignment of the amino acid sequences of three CtUGT from C.tinctorius and other plant UGTs.
VvGT5 (GenBank accession number BAI22846.1 from Vitis vinifera), VvGT6 (BAI22847.1 from V. vinifera), SbUGT (KP183919.1 from Scutellaria baicalensis), MaUGT (A0A096SRM5.1 from Maize), PlUGT (AFI71901.1 from Paeonia lactiflora), GmUGT (BAR88078.1 from Glycine max), PoUGT (ACB56926.1 from Pilosella officinarum), NsUGT (XP_009766439.1 from Nicotiana sylvestris), PmUGT (XP_008229635.1, from Prunus mume), UGT73AE1 (AJT58578.1 from C. tinctorius). All of them are flavonoid glycosyltransferases. Identical amino acids are shown in white on a purple background. The black box indicates the conserved region of plant secondary product glycosyltransferases (PSPG motif).
Table 1.
Identification of UGT genes in safflower by transcriptome sequencing.
Fig 4.
Microarray expression abundance and HCL cluster at different stages of petals for the yellow and white line.
(I) 10 days before blooming. (II) 5 days before blooming. (III) blooming days. (IV) 2 days after blooming.
Fig 5.
Predicted protein tertiary structure of UGT from safflower.
(A) CtUGT3. (B) CtUGT16. (C) CtUGT25.
Table 2.
Primer of UGTs.
Fig 6.
Expression pattern of three UGT genes and relative content changes of flavonoid compounds in C.tinctorius treated with MeJA for 0, 3, 6, and 12 h.
Primary axis: relative expression level. Secondary axis: relative content of compounds. Expression levels were quantified by quantitative PCR. The level of each gene is relative to that of 60 s reference. Each data point is the average of three biological repeats. Error bars indicate SD. A, C, and E are yellow line. B, D and F are white line. A and B are CtUGT3, C and D are CtUGT16, E and F are CtUGT25.
Table 3.
The full set of correlation coefficients between UGTs and flavone glycoside compounds.