Fig 1.
CONSORT Flow Diagram.
Fig 2.
mDA neurons derived from hESCs were treated with N-acetyl cysteine at 10 μM for 3 days before challenge with rotenone (15nM and 30 nM) for 24 hours.
TH+ neurons were quantified after cells were fixed and stained. Data was normalized as percentage of surviving mDA neurons compared to untreated control (100%). Treatment groups were compared with rotenone only groups using unpaired t-test. * p<0.05, ** p<0.01.
Table 1.
Demographics for the study subjects per group.
Table 2.
Mean pre- and post-Tx dopamine transporter binding measures.
Table 3.
Mean pre-to-post-Tx differences in dopamine transporter binding.
Fig 3.
Pre and post NAC DaTscans (left and right respectively) from one particularly responsive patient (A) shows a substantial increase in dopamine transporter binding in the basal ganglia (arrows). We also present (B) the paired t test results overlaid onto a standard MRI template using SPM8 software showing significantly greater binding (p<0.05) post NAC in the basal ganglia.
Table 4.
Mean pre- and post-Tx UPDRS Total Score.
Table 5.
Significant predictors of UPDRS Total Score.