Fig 1.
Ribosomal DNA structure (after Singer and Berg, 1991).
Table 1.
WGS contigs and annotated sequences used to verify the chicken ribosomal cluster assembly.
Table 2.
The rejected sequences of chicken rDNA.
Fig 2.
Localization of assembled rRNA gene cluster fragments on chicken mitotic chromosomes.
FISH with (A) 5’ETS–18S rDNA fragment probe, (D) ITS1–5.8S rDNA fragment probe, (B, E) WAG137G04 BAC probe, which contains NOR, marker of GGA16, (C, F) merge. A, D–green fluorescence; B, E–red fluorescence; C, F–merge. Chromosomes are counterstained with DAPI (blue). Bar – 5 μm.
Table 3.
The list of primers for amplification of target regions of the assembled ribosomal cluster sequence.
Table 4.
GenBank representation of ITS1 and ITS2 sequences.
Fig 3.
The chicken rRNA gene cluster structure and features.