Table 1.
The list of primers used for whole genome sequencing of penguin adenoviruses.
Fig 1.
Putative genetic content of penguin adenovirus.
The range of entire genome lengths of penguin adenoviruses were 24,630–24,662 bp, and the putative sialidase gene was not detected in the penguin adenovirus genomes. The genomic lengths are indicated at5,000 bp intervals.
Table 2.
Summary of deletions of nucleotides and their position in the entire sequence of Chinstrap penguin adenovirus (CSPAdV) and Gentoo penguin adenovirus (GPAdV).
Fig 2.
Phylogenetic analysis of amino acid sequences of penguin adenoviral hexon.
The phylogenetic tree, based on entire hexon genome sequences of penguin adenoviruses, was generated using the Bayesian and maximum likelihood (ML) method. The first number indicates the Bayesian posterior probability, and the second number indicates the ML bootstrap value as a percentage. Scale bars indicate the number of nucleotide substitutions per site.
Fig 3.
Phylogenetic relationships within the genus Siadenovirus based on partial DNA polymerase sequences (a) inferred from nucleotide alignment and (b) from amino acid alignment.
The phylogenetic tree was obtained with a Bayesian inference of phylogeny by the MrBayes v3.1.2 software.
Table 3.
PCR positivity rate for penguin adenovirus in Chinstrap penguins (CSP), Gentoo penguins (GP), and Adelie penguins (GP), Antarctica, 2008–2013.
Table 4.
Detection of the adenoviral genome in tissue and fecal samples collected from penguins infected with adenovirus by PCR.