Fig 1.
Comparison of flowering between ‘VcFT-Aurora’ and ‘Aurora’ plants.
a, Continuous flowering of non-vernalized one-year old ‘VcFT-Aurora’ (left) alongside ‘Aurora’ plants with no flowers (right). Arrows show flowers. b, Floral bud formation on the new shoots of ‘VcFT-Aurora’. c, Vegetative buds, but not floral buds, appeared on new shoots of ‘Aurora’. d, Fully vernalized ‘VcFT-Aurora’ flowered normally.
Table 1.
Statistic analysis of blueberry transcriptome reference developed using Trinity.
Nt: nucleotide.
Table 2.
Differential expression of blueberry flowering pathway genes in leaf, bud, and flower tissues of ‘Legacy’.
Fig 2.
Comparison of transcript expression profiles between ‘VcFT-Aurora’ and ‘Aurora’ to identify differentially expressed genes (a, b) and isoforms (c, d) in leaf tissues. a, c, MA plot for differential expression analysis generated by EdgeR: for each gene or isoform, the log2(fold change) (log2(Aurora/VcFT-Aurora)) between the two samples is plotted (A, y axis) against the gene’s log2(average expression) in the two samples (M, x axis). b, d, Volcano plot reporting false discovery rate (−logFDR, y axis) as a function of log2(fold change) between the samples (logFC, x axis). Genes or isoforms that are identified as significantly differentially expressed at most 0.1% FDR are colored in red.
Fig 3.
RT-PCR analysis of differentially expressed transcripts in leaf tissues of non-transgenic ‘Aurora’ (WT) and transgenic ‘VcFT-Aurora’ (Tr).
VcACTIN is the internal control. M: 1 Kb ladder.
Table 3.
Differential expression of blueberry flowering pathway genes in ‘VcFT-Aurora’ leaves and their putative pathways.
Fig 4.
Flowering pathways in blueberry.
Arrows: promotion. T-signs: inhibition. Solid lines: results of this study. Dashed lines: proposed correlation.