Table 1.
Patient characteristics of entire study population.
Table 2.
Baseline characteristics of human allograft biopsies according to the histopathological grading.
Table 3.
Clinical outcome data.
Fig 1.
Allograft rejection enhances cellular infiltration.
Quantity of CD68 (A), Ki67 (B), and MHCII-HLA-DR (C) positive cells in renal allograft biopsies dependent on underlying histopathological category. # p < 0.05 vs. normal histology. ABMR = antibody mediated rejection, TCMR- = T-cell mediated rejection without arteritis; TCMR+ = T-cell mediated rejection with arteritis; IFTA = interstitial fibrosis/tubular atrophy.
Fig 2.
Compartment-specific macrophages expression.
Compartment-specific expression (peritubular (A), glomerular (B), perivascular (C)) of CD68 positive macrophages in renal allograft biopsies dependent on histological grading. # p < 0.05 vs. normal histology; * p < 0.05 vs TCMR without arteritis. D: CD68 expression in a representative biopsy (TCMR with arteritis).
Fig 3.
Compartment-specific cellular proliferation.
Compartment-specific expression (peritubular (A), glomerular (B), perivascular (C)) of Ki67 positive cells in renal allograft biopsies dependent on histopathological grading. # p < 0.05 vs. normal histology;; * p < 0.05 vs TCMR without arteritis. D: left-hand side: Ki67 expression in a representative biopsy (TCMR with arteritis); right-hand side: representative example of a Ki67 (green) /CD68 (red) costaining (orange arrow).
Fig 4.
Compartment-specific antigen expression.
Compartment-specific expression (peritubular (A), glomerular (B), perivascular(C)) of MHCII-HLA-DR positive cells in renal allograft biopsies dependent on histopathological grading. # p < 0.05 vs. normal histology. D: Representative slide of a CD68-MHCII-HLADR costaining in a TCMR with arteritis biopsy.
Fig 5.
Consequences of macrophages infiltration for renal function and graft survival.
A: Association of CD68+ cell infiltration (below vs. above the median of macrophage infiltration) with corresponding serum creatinine values until 36 months after transplantation. # p < 0.05. B: ROC curve analysis on impact of CD68 infiltration for need for dialysis initiation (12months/36 months after transplantation). C: Kaplan-Meier curve for corresponding overall graft survival (below vs. above the median)