Fig 1.
Summary of workflow for case selection and annotation using the NEPTUNE Digital Pathology repository.
Fig 2.
Digital review and annotation of whole slide images in the NEPTUNE digital pathology repository.
A) Example of multiple levels visualized at the same time. The software allows overlapping and orientation of the sections to facilitate multilevel reconstruction and representation of the biopsy levels. B) Digital annotation of whole slide images. Two levels are shown in alignment. Glomeruli in the left panel are annotated in red. In a subsequent level (right panel), two of the red-annotated glomeruli are still present (3 and 4), while one has disappeared (6). Three additional glomeruli, including one adjacent to glomerulus 3 and 6 have appeared in the deeper level (annotated in blue).
Table 1.
Number of annotated versus reported glomeruli stratified by diagnosis.
Table 2.
Difference in glomerular count of number of annotated versus reported glomeruli stratified by number of glomeruli.
Fig 3.
Digital annotation of whole slide images improves accuracy of glomerular enumeration.
(A,B) Scatter plot of annotated glomerular number versus reported glomerular number by diagnosis in cases reporting (A) total number of glomeruli or (B) average number of glomeruli per level. Dotted diagonal line shows where annotated = reported, and highlights the increase in number of glomeruli found by annotation. (A: n = 139; B: n = 138). (C,D) Box plot of annotated glomerular number and reported total glomerular number stratified by diagnosis in cases reporting (C) total number of glomeruli (n = 139) or (D) average number of glomeruli (n = 139).
Table 3.
Number of annotated versus reported percent globally sclerotic (%GS) stratified by diagnosis.
Table 4.
Difference in %GS glomeruli between annotated versus reported stratified by number of glomeruli.
Fig 4.
Digital annotation of whole slide images modestly improves accuracy of global glomerular sclerosis enumeration.
(A,B) Box plot of annotated percent (%) globally sclerotic glomeruli and reported %globally sclerotic glomeruli in cases reporting (A) total number of glomeruli (n = 139) or (B) average number of glomeruli (n = 138). (C,D) Box plot of difference between number of annotated and reported % globally sclerotic glomeruli stratified by % globally sclerotic glomeruli in cases reporting (C) total number of glomeruli (n = 139) or (D) average number of glomeruli (n = 138).