Table 1.
Primers for real-time RT-PCR amplification and lengths of amplified DNA.
Fig 1.
Mean monthly temperature (mean, maximum and minimum), heat units and precipitation during cotton growth period in 2013 to 2014.
Heat units were calculated from maximum and minimum temperatures as ∑[(max. temp. + min. temp.)/2–15°C].
Fig 2.
Effects of soil salinity on chlorophyll components in the youngest fully expanded main-stem leaf (MSL).
(a) Chl a content. (b) Chl b content. (c) Chl(a+b) content. (d) carotend content.
Fig 3.
Effects of soil salinity on chlorophyll components in the subtending leaf of cotton boll (LSCB).
(a) Chl a content. (b) Chl b content. (c) Chl(a+b) content. (d) Carotend content.
Fig 4.
Effects of soil salinity on photosynthetic parameters in the youngest fully expanded main-stem leaf (MSL) and the subtending leaf of cotton boll (LSCB).
(a) Photosynthetic parameters of the MSL in 2013. (b) Photosynthetic parameters of the MSL in 2014. (c) Photosynthetic parameters of the LSCB in 2013. (d) Photosynthetic parameters of the LSCB in 2014.
Fig 5.
Effects of soil salinity on carbohydrates content in the youngest fully expanded main-stem leaf (MSL).
(a) Soluble sugar content. (b) Sucrose content. (c) Starch content. (d) Sucrose/Starch.
Fig 6.
Effects of soil salinity on carbohydrates content in the subtending leaf of cotton boll (LSCB).
(a) Soluble sugar content. (b) Sucrose content. (c) Starch content. (d) Sucrose/Starch.
Fig 7.
Effects of soil salinity on SPS and SuSy activities in the youngest fully expanded main-stem leaf (MSL).
(a) SPS activity. (b) SuSy activity.
Table 2.
F values of ANOVA of the effect of year, salinity level, cultivar, and their interactions with sucrose phosphate synthase (SPS) and sucrose synthase (SuSy) peak values in 2013 and 2014.
MSL: the youngest fully expanded main-stem leaf, LSCB: the subtending leaf of cotton boll.
Fig 8.
Effects of soil salinity on SPS and SuSy activities in the subtending leaf of cotton boll (LSCB).
(a) SPS activity. (b) SuSy activity.
Fig 9.
Effects of soil salinity on Inv activity in the youngest fully expanded main-stem leaf (MSL) and the subtending leaf of cotton boll (LSCB).
(a) Inv activity in the MSL. (b) Inv activity in the LSCB.
Table 3.
Relationship between carbohydrate content and sucrose metabolism enzymes in the youngest fully expanded main-stem leaf (MSL) and the subtending leaf of cotton boll (LSCB) for cultivars CCRI-79 and Simian 3 for three salinity levels in 2013 and 2014.
MSL: the youngest fully expanded main-stem leaf, LSCB: the subtending leaf of cotton boll.
Table 4.
Relationship between photosynthetic rate (Pn) and Chl and sucrose-metabolizing enzymes in the youngest fully expanded main-stem leaf (MSL) and the subtending leaf of cotton boll (LSCB) for cultivars CCRI-79 and Simian 3 for three salinity levels in 2013 and 2014.
Table 5.
Correlation coefficients between boll weight and photosynthetic rate (Pn) carbohydrate contents, and sucrose-metabolizing enzymes in the youngest fully expanded main-stem leaf (MSL) and the subtending leaf of cotton boll (LSCB) for cultivars CCRI-79 and Simian 3 for three salinity levels in 2013 and 2014.
Fig 10.
Effects of soil salinity on relative expression levels of sps isozyme genes in the subtending leaf of cotton boll (LSCB).
(A) Relative expression levels of sps isozyme genes for CCRI-79. (B) Relative expression levels of sps isozyme genes for Simian 3.
Fig 11.
Effects of soil salinity on relative expression levels of sus isozyme genes in the subtending leaf of cotton boll (LSCB).
(A) Relative expression levels of sus isozyme genes for CCRI-79. (B) Relative expression levels of sus isozyme genes for Simian 3.