Table 1.
Sequences of primers and probe used for HLA-B*15:02 screening by AS-PCR and DDB.
Fig 1.
(a) Electrophoresis was applied by using 2.5% agarose gel with 100V for 30 minute. A control product was 301 bp long while the specific PCR product was 137 bp long. The alleles with PCR product included HLA-B*15:02 (No. 1 and 8), B*15:21 (No. 2), B*15:25 (No. 3), B*15:11 (No. 4), B*15:32 (No. 5), B*15:01 (No. 6), B*15:31 (No. 7), and B*46:01 (No. 4, 8). HLA-B*15:13 (No. 9) and other common alleles (No. 10–16) gave negative results in the PCR step. (b) The samples analyzed in the PCR step (No. 1–9) were subsequently examined by DDB. (NTC: “no template control”).
Fig 2.
A diagram of test results and their representative alleles.
A diagram demonstrated the number of samples grouped by their results and the alleles which could give each result. In the AS-PCR step, all samples were tested: 82 gave negative results and were reported as negative for screening, following which 73 positive AS-PCR samples were then tested in the DDB step, giving 47 negative and 26 positive results. The only falsely screened result was from HLA-B*15:31.
Table 2.
Comparison of test characteristics of HLA-B*15:02 screening methods.