Fig 1.
Histopathology of POWV-infected and uninfected Ixodes scapularis feeding sites, 3 and 6 hpi.
Biopsies from tick feeding sites were sectioned and stained with H&E as described in Materials and Methods section. (A). POWV-infected section at 3 hpi. (B). Uninfected section at 3 hpi. (C). POWV-infected section at 6 hpi. (D). Uninfected section at 6 hpi. In panel A, the red oval indicates the area with high levels of cellular infiltrates. In panels A, B, and D, the arrow is pointing to the tick hypostome. In panel C, the arrow indicates where the tick hypostome was located prior to being dislodged during sectioning. Scale bars represent 100 μm.
Fig 2.
Histopathology of POWV-infected and uninfected Ixodes scapularis feeding sites, 12 and 24 hpi.
Biopsies from tick feeding sites were sectioned and stained with H&E as described in Materials and Methods section. (A). POWV-infected section at 12 hpi. (B). Uninfected section at 12 hpi. (C). POWV-infected section at 24 hpi. (D). Uninfected section at 24 hpi. In panels A, B, C, and D, the arrow is pointing to the tick hypostome. Scale bars represent 100 μm.
Fig 3.
Immune cells detected at the POWV-infected Ixodes scapularis feeding sites, 3 and 6 hpi.
(A). Images of skin at the POWV-infected tick feeding site where macrophages are shown in orange and POWV-infected cells are shown in red. The F4/80 marker was used for macrophage detection. (B). Images of skin at the POWV-infected tick feeding site where fibroblasts are shown in orange and POWV-infected cells are shown in red. The vimentin marker was used for fibroblast detection. Scale bars represent 10 μm. DAPI (4’,6-diamidino-2-phenylindole) was used for nuclear counterstaining. Open arrowheads indicate POWV-infected macrophages or POWV-infected fibroblasts. Closed arrowheads indicate other cells that are POWV-infected but that are not macrophages or fibroblasts.
Fig 4.
Immune cells detected at the POWV-infected Ixodes scapularis feeding sites, 12 and 24 hpi.
(A). Images of skin at the POWV-infected tick feeding site where macrophages are shown in orange and POWV-infected cells are shown in red. The F4/80 marker was used for macrophage detection. (B). Images of skin at the POWV-infected tick feeding site where fibroblasts are shown in orange and POWV-infected cells are shown in red. The vimentin marker was used for fibroblast detection. Scale bars represent 10 μm. DAPI (4’,6-diamidino-2-phenylindole) was used for nuclear counterstaining. Open arrowheads indicate POWV-infected macrophages or POWV-infected fibroblasts. Closed arrowheads indicate immune cells that are POWV-infected but not macrophages or fibroblasts.