Table 1.
Chromosome pairing at metaphase I in the pollen mother cells of hexaploid triticale lines.
Fig 1.
Meiotic metaphase I pairing analysis of hexaploid triticale lines.
(a) Line K14-488-1, 2n = 42 = 1 II (rod) + 20 II (ring). (b) Line K14-489-2, 2n = 42 = 2 I + 4 II (rod) + 16 II (ring). (c) Line K14-491-2, 2n = 42 = 4 I + 3 II (rod) + 16 II (ring). (d) Line K14-547-1, 2n = 42 = 1 I + 1 II (rod) + 18 II (ring) + 1III (arrow).
Table 2.
Chromosome constitutions of six hexaploid triticale lines.
Fig 2.
GISH identification of hexaploid triticale lines at mitotic metaphase (a–c), and meiotic metaphase I and anaphase I (d–f).
The rye genomic DNA was used as probes for in situ hybridization. Chromosomes in red and yellow-green are wheat and rye chromosomes, respectively. (a) Line K14-488-1, 2n = 42 = 14R +28W. (b) Line K14-489-2, 2n = 42 = 12R +30W. (c) Line K14-491-2, 2n = 42 = 12R +30W. (d) Line K14-493-1, 2n = 42 = 14R +28W, rye chromosomes with 1 rod and 6 ring bivalents were labeled. (e) Line K14-489-2, 2n = 42 = 12R +30W, rye chromosomes with 4 rod and 2 ring bivalents were labeled. (f) Line K14-491-2, rye chromosomes showed regular segregation at anaphase I.
Fig 3.
FISH karyotypes of hexaploid triticale lines using pSc119.2 and pTa535 as probes.
Probes pSc119.2 and pTa535 signals were pseudo-colored as green and red in the FISH patterns, respectively. (a) Line K14-488-1 had complete 28 A/B and 14 R chromosomes. (b) Line K14-489-2 had complete 28A/B and 1D (arrows), 2R, 3R, 4R, 5R, 6R and 7R chromosomes. (c) Line K14-491-2 had 12 A (1A-6A), 14 B (1B-7B), 12 R (1R-3R, 5R-7R), and 1D and 3D (arrows) chromosomes. (d) Line K14-493-1 had complete 28 A/B and 14 R chromosomes. (e) Line K14-545-2 had complete 28 A/B and 14 R chromosomes. (f) Line K14-547-1 had 14 A (1A-7A), 12 B (1B-5B, 7B), 14 R, and a pair of 6BL/2DS translocation chromosomes (arrows).
Fig 4.
HMW-GS variations in hexaploid triticale lines and their parents.
1 Zhongsi828, 2 PHW-SA, 3 K14-488-1, 4 K14-491-2, 5 K14-493-1, 6 K14-545-2, 7 K14-547-1, 8 K14-489-2. Black arrows indicate position of inherited HMW-GS subunit of Zhongsi828. Red arrow indicates a specific band was only expressed in K14-488-1.
Table 3.
Infection types of hexaploid triticale lines and their parents for stripe rust with a mixture of races at seedling and adult plant stages.