Fig 1.
OBE3 gene structure and mutant phenotypes.
(A) Structure of the OBE3 gene. The upstream region used for the complementation is shown in green. (B-D) Phenotypes of the denoted genotypes of 10-day-old seedlings (B), shoots (C), and flowers (D). Scale bars: 1 mm (B, D), 2 cm (C).
Table 1.
obe3-2 enhances the meristem defects of weak and intermediate wus alleles.
Table 2.
Flower phenotypes of obe3-2 wus-1, obe3-2 wus-7 and obe3-2 wus-6.
Fig 2.
Genetic combinations of obe3-2 and obe4-2.
(A) Plants of obe3-2 and obe4-2 single mutants are indistinguishable from wild type. (B) obe3-2 obe4-2 seedlings that did not develop further. (C) obe3-2 obe4-2/+ plants displayed two phenotypic classes at 60 DAG. (D) obe4-2 wus-6 plant displayed wus-6-like shoot formation at 60 DAG. (E) obe4-2 wus-6 plant produced complete flowers and wus-1 like flowers. Scale bars: 2 cm (A, C and D), 1 mm (B and E).
Fig 3.
Changes of transcripts in obe3-2 and obe4-2.
(A) Transcript levels of 7-day-old seedlings as indicated. Error bars represent SE. (B) After induction of OBE3 overexpression, mRNA levels of WUS are increased, whereas mRNA levels of CLV3, STM, and ARR7 are unchanged in 7-day-old seedling. Error bars represent SD. (C) pWUS:GUS expression in 6-day-old obe3-1 obe4-1/+ seedlings is confined to the OC as in the wild type. (D) WUS mRNA is undetectable by in situ hybridization in obe3-1 obe4-1/+ floral meristems of 30-day-old plants. (E) WUS overexpression upregulates OBE3 and OBE4 mRNA levels in 7-day-old seedlings. ARR7 expression is used as a control. Error bars represent SD. Relative mRNA levels compared to the mock control are shown.*,p<0.05, calculated from Cp’ values; ***, p<0.001, calculated from Cp’ values.
Fig 4.
p35S:cOBE3 expression partially suppresses wus-1 defects.
(A) Phenotypes of segregating seedlings in the progeny of a p35S:cOBE3 wus-1/+ mother plant. (B) p35s:cOBE3 wus-1 plants produce wus-1-like flowers. (C) Model for WUS-OBE3 interaction. Scale bars: 1 mm.