Table 1.
Strains and virus used in this study.
Table 2.
Characteristics of the underground brines.
Fig 1.
Standard curve for ten-fold serial dilutions of virus SNJ1.
The Ct values are plotted against the corresponding log of the plaque-forming units per ml. R2 = 0.998; the amplification efficiency of qPCR is 99.27%.
Fig 2.
Melting curve analysis of qPCR products.
The graph represents qPCR reactions plotted as the negative first derivative of the temperature versus fluorescence (-dRFU/dT) against temperature, representing a function of the decrease in SYBR Green I fluorescence due to increasing temperature.
Fig 3.
Population dynamics of SNJ1 determined by plaque assay and qPCR.
The cells of Natrinema sp. J7-2 were infected by halovirus SNJ1 with an MOI of 50 for 1.5 h, then samples were taken from the culture system at indicated times, and the titers of samples were detected by plaque assay and qPCR. (○) viral titer determined by qPCR; (□) viral titer determined by plaque assay.
Fig 4.
Standard curves for ten-fold serial dilutions of halovirus SNJ1 added to natural water samples.
The Ct values are plotted against the corresponding log PFU/ml of halovirus SNJ1. R2 >0.98 and amplification efficiency was more than 83% in all cases.
Table 3.
The precision testing of quantitative real-time PCR.