Fig 1.
Effects of 17-DMAG (17D) on mean arterial blood pressure (MAP) in LPS induced endotoxemic rats.
17-DMAG (17D; 5 mg/kg) was given 20 h or 0 min prior to LPS initiation (30 mg/kg/4 h). Quercetin (Q; 300 mg/kg, i.p.), an HSP70 inhibitor, was given 6 h prior to LPS initiation. Values are expressed as mean±SEM. Animal number in each group is shown in the parenthesis. *P < 0.05 vs. sham group, # P < 0.05 vs. LPS group.
Fig 2.
Effects of 17-DMAG (17D) on alanine aminotransferase (ALT) (A), lactate dehydrogenase (LDH) (B), creatine phosphokinase (CPK) (C), and creatinine (CRE) (D) in rats with endotoxemia induced by LPS administration.
Q: quercetin; Values are expressed as mean±SEM. Animal number in each group is shown in the parenthesis. *P < 0.05 vs. sham group; #P < 0.05 vs. LPS group; +P < 0.05 vs.17D+LPS group.
Fig 3.
Effects of 17-DMAG (17D) on LPS-induced cardiac caspase 3 activity.
Q: quercetin; Values are expressed as mean±SEM. Animal number in each group is shown in the parenthesis. *P < 0.05 vs. sham group; #P < 0.05 vs. LPS group; +P < 0.05 vs.17D+LPS group.
Fig 4.
Effects of 17-DMAG (17D) on the plasma concentrations of nitric oxide (NO) metabolites (A), TNF-α (B), IL-6 (C), and phosphorylated NF-κB p65 (pp65) protein expression in lungs (D) after rats subjected to LPS administration.
The level of TNF-α was detected 2 h after LPS initiation; NO metabolites, IL-6, and pp65 levels were detected after 6 h. Q: quercetin; Values are expressed as mean±SEM. Animal number in each group is shown in the parenthesis. *P < 0.05 vs. sham group; #P < 0.05 vs. LPS group. +P < 0.05 vs.17D+LPS group.
Fig 5.
Effects of 17-DMAG (17D) on the levels of superoxide anion production (A) and reduced glutathione (B) in hearts of rats 6 h after LPS administration.
Q: quercetin; Values are expressed as mean±SEM. *P < 0.05 vs. sham group; #P < 0.05 vs. LPS group.
Fig 6.
Effects of 17-DMAG (17D) on prothrombin time (A), platelet count (B) and PAI-1 protein expression in lungs (C) 6 h after LPS administration.
Q: quercetin; Values are expressed as mean±SEM. Animal number in each group is shown in the parenthesis. *P < 0.05 vs. sham group; #P < 0.05 vs. LPS group.
Fig 7.
Effects of 17-DMAG (17D) on HSP70 protein expression in hearts (A), lungs (B), and livers (C) of rats 6 h after LPS administration.
Q: quercetin; Values are expressed as mean±SEM. *P < 0.05 vs. sham group; #P < 0.05 vs. LPS group; +P < 0.05 vs.17D+LPS group.
Fig 8.
Effects of 17-DMAG (17D) on iNOS protein expression in hearts (A), lungs (B), and livers (C) of rats 6 h after LPS administration.
Q: quercetin; Values are expressed as mean±SEM. *P < 0.05 vs. sham group; #P < 0.05 vs. LPS group; +P < 0.05 vs.17D+LPS group.
Fig 9.
Effects of 17-DMAG (17D) on HO-1 protein expression in hearts (A), lungs (B), and livers (C) of rats 6 h after rats subjected to LPS administration.
Q: quercetin; Values are expressed as mean±SEM. #P < 0.05 vs. LPS group.
Fig 10.
Effects of 17-DMAG (17D) on histologic studies of lung sections in the sham (A), 17D (B), LPS (C), 17D+LPS (D), LPS+17D (E), and 17D+Q+LPS groups (F).
Q: quercetin; Sections were stained with hematoxylin and eosin, each X400 (original magnification). Scale bar = 50 μm. Arrows indicate infiltration of the inflammatory cells.
Fig 11.
Effects of 17-DMAG on survival rate of rats after LPS administration.
17-DMAG (17D; 5 mg/kg) was given 20 h or 0 min prior to LPS initiation (30 mg/kg/4 h). Quercetin (Q; 300 mg/kg, i.p.), an HSP70 inhibitor, was given 6 h prior to LPS initiation. Data are shown as mean± SEM. *P < 0.05 vs. the sham group; #P < 0.05 vs. the LPS group, +P < 0.05 vs.17D+LPS group; n = 5–20.