Table 1.
PCR primers pairs used for the amplification of 18S rRNA gene fragments from microalgae under study and accession numbers amplified sequences published in NCBI Gene Bank.
Fig 1.
Growth response of microalgal species isolates observed on 18th day (a) measured as OD at 730 nm and (b) measured as cell density per ml. The values are mean of three replicates and error bar shown are 5% of the mean value.
Fig 2.
Accumulation of total lipid in isolates of microalgal sp. over 18th day of cultivation, (a) Direct gravimetric estimation using modified Bligh & dyer method; Total lipid expressed as percentage (w/w) in dried biomass. (b) Quantified by the Nile red fluorescence method. Data were the mean values and standard error of three replicates.
Table 2.
Growth kinetics, biomass, biomass productivity, lipid content, and lipid productivity of Scenedesmus dimorphus isolates.
Table 3.
Growth kinetics, biomass, biomass productivity, lipid content, and lipid productivity of Scenedesmus quadricauda isolates.
Fig 3.
Cumulative occurrence of five important fatty acids commonly found in biodiesel in total lipids in microalgal species.
Table 4.
Comparison of biomass productivity, lipid content and productivity and fatty acid composition of some microalgae reported in similar studies with S. dimorphus isolates Sd12 and Sd1.
Fig 4.
Cells and colonies of microalgae species as observed under light microscope (100X), (a) Scenedesmus quadricauda, (b) Scenedesmus dimorphus and (c) Chlorella sp.
Fig 5.
Scanning electron micrographs of Scenedesmus quadricauda (a), (b) Scenedesmus dimorphus and (c) Chlorella sp. a: Magnification 3500×; b: Magnification 4000×; c: Magnification 4500×.
Fig 6.
Amplified 18S rRNA gene fragments using P73-P47, Chloro F &R, CV1-CV2, CV3-CV4 primer pairs from genomic DNA of Scenedesmus quadricauda (lane 2,3,4, 5), Chlorella sp. (Lane 6,7,8,9) Scenedesmus dimorphus (Lane 10,11,12,13) Lane 1 is 100 bp ladder.
Fig 7.
Phylogenetic trees of microalgae (a) Scenedesmus quadricauda, (b) Scenedesmus dimorphus and (c) Chlorella sp. inferred from alignment of their 18S rRNA gene fragment sequences and ten most similar sequences of same genus retrieved from NCBI database. The query sequence of respective species of this study designated as name of the specie followed by F4.