Fig 1.
Saprophytic life cycle of Alternaria invading its host plants.
Fig 2.
Tobacco leaves showing different brown spot symptoms.
(A) Typical brown spot lesions on tobacco leaves, from which CX1 was isolated. (B) Brown spots lesions on sunburned tobacco leaves, which served to isolate CX2.
Fig 3.
Phylogenetic relationship between CX1 and CX2.
(A) Phylogenetic relationship constructed by MEGA5 based on ITS sequences of CX1, CX2, A. longipes EGS30-033, A. alternata SDHeze-9, A. tenuissima CSPF5 and A. brassicicola Ab4UP. (B) Phylogenetic relationship constructed by MEGA5 based on large co-linear sequence among CX1, CX2, A. alternata atcc11680, A. longipes bmp0313 and A. tenuissima bmp0304.
Table 1.
Statistics for the assembled genome sequences of A. longipes cx1 and A. alternata cx2.
Fig 4.
Synteny dotplot of A. longipes cx1 (y-axis) and A. alternata cx2 (x-axis).
Regions of homology are plotted as diagonal lines.
Table 2.
A. longipes cx1 and A. alternata cx2 genome features.
Fig 5.
Phylogenetic relationship among A. longipes cx1, A. alternata cx2, F. oxysporum, M. oryzae, A. nidulans, L. maculans, P. teres, P. nodorum, A. abrassicicola and A. alternata SRC1lrK1f.
The estimates of divergence time and its interval based on sequence identity are indicated at each node. The red dot on branches means divergence time has been adjusted by fossil evidence.
Fig 6.
Venn diagram showing the number of unique and shared gene families among A. longipes cx1, A. alternata cx2, A. brassicicola and A. alternata SRC1lrK1f.
Table 3.
Three gene families specific to A. longipes cx1 and A. alternata cx2 have different gene copies between these two strains.
Fig 7.
NRPS-PKS homologs in A. longipes cx1 and A. alternata cx2.
(A) TAS1 homologous gene in A. longipes cx1 (AL_scaffold88_6305) and A. alternata cx2 (AA_scaffold3_8872) might be required for TeA synthesis. (B) PKSH homologous gene in A. longipes cx1 (AL_scaffold86_4815) and A. alternata cx2 (AA_scaffold4_10387, AA_scaffold12_4792). (C) PKSJ homologous gene in A. longipes cx1 (AL_scaffold19_7914) and A. alternata cx2 (AA_scaffold4_10765, AA_scaffold21_8045). The direction of arrow indicates N-terminal.
Table 4.
Genes related to KEGG plant-pathogen interaction.
Fig 8.
Distribution of carbohydrate-active enzymes gene families in A. longipes cx1, A. alternata cx2, A. abrassicicola and A. alternata SRC1lrK1f.