Fig 1.
Caprellid species used in the present study.
Left: field populations inhabiting marinas of Southern Spain. Right: Lateral view figures of male and female specimens of each species.
Fig 2.
Total area occupied by the gut content in the whole digestive tract of Caprella equilibra and C. scaura.
‘Marina’ includes data of the field populations directly fixed after collection. Treatments represent the different feed provided in laboratory conditions: Artemia nauplii, Detritus, Phytoplankton, All feed provided together (All), and no feed provided (NF). The bars indicate the percentage of each item observed in the gut under the microscope (Crustacea, Phytoplankton and Detritus). The Crustacea are represented by copepods in specimens from the field populations of the marinas and Artemia in those maintained in laboratory conditions fed with Artemia.
Table 1.
Results of the two-way ANOVA for the percentage of gut contents during the feeding experiment.
Table 2.
Main lipid classes (μg/100μg DW) of the three types of feed used in the experiment as diets (A: Artemia, P: Phytoplankton, D: Detritus), the caprellids (Eq: C. equilibra, Sc: C. scaura) collected from marinas (used as reference values) and the caprellids fed either Artemia, phytoplankton or detritus during 12 days in laboratory conditions (treatments).
Table 3.
Fatty acid composition (expressed as % of the total identified fatty acids) of the three types of feed used in the experiment as diets (A: Artemia, P: Phytoplankton, D: Detritus), the caprellids (Eq: C. equilibra, Sc: C. scaura) collected from marinas (used as reference values) and the caprellids fed either Artemia, phytoplankton or detritus during 12 days in laboratory conditions (treatments).
Fig 3.
Principal Component Analysis (PCA) plot based on the Lipid Classes (LCs) composition.
LCs were analysed for the three types of feed used in the experiment as diets (A: Artemia, P: Phytoplankton, D: Detritus), the caprellids (Eq: C. equilibra, Sc: C. scaura) collected from marinas (used as reference values) and the caprellids fed either Artemia, phytoplankton or detritus for 12 days under laboratory conditions (EqA, EqP, EqD, ScA, ScP, ScD). Subscript numbers (1,2,3) correspond to each replicate value.
Table 4.
Summary of the two-way PERMANOVA results examining the lipid classes and fatty acids of caprellids fed either Artemia (A), phytoplankton (P) or detritus (D) during 12 days in laboratory conditions.
Table 5.
Summary of the two-way ANOVA results examining the selected lipid classes and fatty acids (those which correlated with axis 1 and 2 of the PCA analysis).
Caprellids were fed either Artemia (A), phytoplankton (P) or detritus (D) during 12 days in laboratory conditions.
Fig 4.
Principal Component Analysis (PCA) plot based on the Fatty Acid (FAs) composition.
FAs were analysed for the three types of feed used in the experiment as diets (A: Artemia, P: Phytoplankton, D: Detritus), the caprellids (Eq: C. equilibra, Sc: C. scaura) collected from marinas (used as reference values) and the caprellids fed either Artemia, phytoplankton or detritus for 12 days under laboratory conditions (EqA, EqP, EqD, ScA, ScP, ScD). Subscript numbers (1,2,3) correspond to each replicate value. Replicates Eq3, EqA3, EqP3 and Sc3 are not included since they were damaged during the chemical procedure and lost. For PERMANOVA analyses, the values of these replicates were filled with the mean values of the other two remaining replicates.
Fig 5.
Survival rate (%) of adult specimens of Caprella equilibra and C. scaura after 12 days.
Specimens were fed with Artemia, phytoplankton or detritus.
Table 6.
Results of the two-way ANOVA for the survival percentage of adult caprellids after the 12 days experiment.
Fig 6.
Survival rate (%) of juvenile specimens newly emerged from the brood pouch of Caprella equilibra and C. scaura after 16 days.
Specimens were fed with Artemia, phytoplankton or detritus.
Fig 7.
Body length (mm) and number of articles in the flagellum of antennae 1 of juveniles of Caprella equilibra and C. scaura after 16 days.
Specimens were fed with Artemia, phytoplankton or detritus. Data for detritus in C. scaura is not provided since all the juveniles died before the end of the experiment.
Table 7.
Results of the two-way ANOVA for the survival percentage of juvenile caprellids after the 16 days experiment.