Fig 1.
Hepatic expression profiles of mRNAs and miRNAs in human liver samples.
Box plots indicate first and third quartiles of expression; the bold line in the box represents the median value; the whiskers represent the range.
Fig 2.
Correlation of mRNA expression levels of pro- and anti-coagulant factors with hepatic transcription factor HNF4A.
Positive and significant correlations between procoagulant (F5, F9, F10, F11, and F12), anticoagulant (SERPINC1, PROC, PROS1, and PROZ) factors and HNF4A mRNA expression. qRT-PCR was performed in total RNA purified from healthy livers (n = 104). Each data point represents an individual liver tissue sample, and a correlation coefficient (r) is shown. The results are presented as Log2 fold change with respect to the normalization standard. The asterisk indicates a statistically significant difference (p < 0.0001).
Fig 3.
Fig 4.
Effect of miRNAs on HNF4A and coagulation factors expression in HepG2.
HepG2 cells were transfected with 100 nM mimic precursors of miR-24, miR-34a or SCR. Protein lysates and total RNA were obtained after 48 h incubation and analyzed. A) Densitometric analysis of HNF4α protein expression transfected with SCR, miR-24, and miR-34a; representative western blot. B)qRT-PCR analysis of HNF4A and coagulation factors mRNA expression. The asterisk indicates a statistically significant difference (P < 0.05) and “ns” indicates a statistically non-significant difference (P > 0.05). Results are mean ± SD of three independent experiments performed in triplicate.
Fig 5.
Linear regression analysis between endogenous mature miRNAs (miR-24and miR-34a) levels and HNF4A mRNA.qRT-PCR were performed in total RNA purified from healthy livers (n = 104).
Statistical significance was taken as p<0.05. The results are presented as Log2 fold change with respect to the normalization standard.
Fig 6.
Expression range of miR-24 and miR-34a with HNF4A mRNA and its downstream targets in healthy livers.
Dot plot diagram of HNF4A mRNA, selected coagulation factors and miR-24 (A) or miR-34a (B) levels in livers. P10 and p90 represent 10th and 90th percentiles of miR-24 (A) and miR-34a (B), respectively. Each data point represents an individual liver tissue sample. The results are presented as fold change with respect to the normalization standard. The asterisk indicates a statistically significant difference (P < 0.05) and “ns” indicates a statistically non-significant difference (P > 0.05).