Table 1.
De novo assembly statistics.
Fig 1.
15V-P4 position in the phylogeny of S. cerevisiae strains.
(A) Neighbor joining phylogenetic tree of 95 strains including 15V-P4 inferred from alignment of conservative chromosome regions. (B) Phylogenetic tree of 29 strains including 15V-P4 inferred from sequences of 807 common genes under the GTR+G model and tested with 500 bootstrap replicates. Branch bootstrap values greater than 95 are indicated. In both trees, strain names are colored according to functional origin. Grey circles highlight either the population group (A) or common functional origin (B). Branch lengths are given in the same scale on both trees. PGC, the Peterhof genetic collection.
Table 2.
Genes absent from the S288C genome but found in 15V-P4.
Fig 2.
Genome coverage across reference for representative strains.
(A) 15V-P4, (B) 25-25, (C) 6P-33G. Dashed lines signify chromosome borders.
Table 3.
Lengths of regions annotated as amplified or deleted in each strain and counts of genes included into each of these regions.
Fig 3.
Distribution of variable sites shown in chromosomal coordinates of S288C.
Green: SNVs compared to S288C. Purple: SNVs compared to 15V-P4. Each chromosome is framed.
Table 4.
Selectable marker mutations in the PGC strains.
Fig 4.
Only PHA2P but not pha2 mutant alleles compensate for the pheA10 phenylalanine auxotrophy.
33G-D373 was transformed with plasmids bearing indicated PHA2 alleles. Series of 5-fold dilutions on synthetic media are shown. Vector, pRS316.
Fig 5.
Cell aggregation phenotypes of strains analyzed correlate with AMN1 and FLO8 alleles.
The scale bar indicates 10 um. Amn1 and Flo8 variants are shown in color (green: associated with “clumping” phenotypes; red and purple: “non-clumping”). Representative microphotographs out of five fields of view of yeast liquid medium cultures in early stationary phase are shown.
Table 5.
Yeast strains used in this work.