Fig 1.
A schematic diagram of the experimental groups and triclosan exposures. Labeled tick marks represent days.
Fig 2.
Triclosan exposure is associated with altered microbial community structure.
(A) Phyla level taxa plot of the most abundant taxa in zebrafish gut microbiomes across exposure groups. (B) Non-metric multidimensional scaling analysis of unexposed (red dots), four-day (blue dots) and seven-day (green dots) exposure group’s microbial communities. Colored ellipses represent the 99.9% confidence interval for standard error of each group. (C) Comparisons of within group Bray-Curtis dissimilarity between groups. (D) Shannon entropy between exposure groups. Significant p-values (p < 0.05) are denoted with an asterisk.
Fig 3.
Triclosan exposure is associated with significant alterations in OTU abundance.
Fold change values for OTUs that were significantly altered in abundance in the (A) four-day, and (B) seven day exposure groups. Genus level taxonomic assignments are provided and corresponding OTU IDs indicated inside parentheses.
Fig 4.
Triclosan exposure is associated with unique indicator OTUs.
Indicator values for significant (p < 0.05, q < 0.20) indicator taxa in (A) unexposed and (B) triclosan exposed fish. The size of each point is proportional to its class-wide relative abundance, and its color is proportional to its class-wide frequency.
Fig 5.
Triclosan exposure is associated with alterations in microbial correlation networks.
Interaction networks for microbial communities in (A) unexposed, (B) four-day exposure and (C) seven-day exposure groups. Network communities (subgraphs) are identified by colored polygons and the vertices (OTUs) that comprise these communities are colored identically to indicate network community membership. Each line represents an abundance correlation between two OTUs. The size of each vertex is proportional to its degree. Edges between different network communities are colored red and those within a community are black. (D) Venn diagram of shared vertices between networks. (E) Degree and (F) betweenness centrality distribution for all vertices in network. *** p < 0.001.
Table 1.
Network Properties.