Table 1.
Distribution of lineages/sublineages of isolates from Tanzania (n = 293).
Table 2.
Descriptive statistics on age of patients in this study.
Table 3.
Distribution of Spoligotype International Types (SIT) in this study.
Table 4.
Description of clusters containing 3 or more isolates in this study, and their worldwide distribution in the SITVIT2 database.
Table 5.
Distribution of phylogenetical lineages vs. demographic characteristics of patients and cities of isolation.
Fig 1.
Distribution map of Mycobacterium tuberculosis lineages in several countries of Africa according to the SITVIT2 database.
Fig 2.
A minimum spanning tree (MST) illustrating evolutionary relationships between M. tuberculosis spoligotypes.
MST constructed on all isolates including the orphan patterns (n = 293) in function of various characteristics. (A)MST based on phylogenetical lineages; (B) MST based on cities of isolation; and (C) MST based on HIV serology. The phylogenetic tree connects each genotype based on degree of changes required to go from one allele to another. The structure of the tree is represented by branches (continuous vs. dashed and dotted lines) and circles representing each individual pattern. Note that the length of the branches represents the distance between patterns while the complexity of the lines (continuous, gray dashed and gray dotted) denotes the number of allele/spacer changes between two patterns: solid lines, 1 or 2 or more changes (thicker ones indicate a single change, while the thinner one indicate 2 changes); gray dashed lines represent 3 changes; and gray dotted lines represent 4 or more changes. The size of the circle is proportional to the total number of isolates in our study, illustrating unique isolates (smaller nodes) versus clustered isolates (bigger nodes). The color of the circles indicates the phylogenetic lineage to which the specific pattern belongs.
Fig 3.
(A) Zoomed part of Spoligoforest tree showing SIT126 tentatively relabeled "EAI3-TZA" and its spoligotype descendants, along with their binary spoligotyping descriptions highlighting the specific absence of spacers 37, 38 and 40; and (B) Geographic distribution maps/Intensity maps of SIT126 and SIT8 (by percentage in country) according to SITVIT2 database before entering this study.