Table 1.
Details for primer sets used in this study.
Fig 1.
Relationship between sample age and recovered sequence length from six collections in reptiles and amphibians.
AMNH: American Museum of Natural History; FMNH: Field Museum of Natural History; KU: University of Kansas Biodiversity Institute; MVZ: Museum of Vertebrate Zoology; ROM: Royal Ontario Museum; SDNHM: San Diego Natural History Museum; UAHC: University of Alabama, Alabama Museum of Natural History Herpetological Collection.
Fig 2.
Sequence recovery in (A) amphibians and (B) reptiles from six collections.
Qualifying barcodes are sequences which are ≥487bp.
Fig 3.
The barcode gap and institutional errors.
Comparison of maximum intraspecific sequence divergence with minimum interspecific sequence divergence for amphibians and reptiles. Points above the 1:1 line indicate that a barcode gap is present; points below the line indicate its absence. Points representing museum errors and re-sequenced specimens came from the Royal Ontario Museum.
Fig 4.
The correspondence between Barcode Index Number (BIN) and species assignment.
The number of BIN matches, splits, merges, and mixes for the four orders in this study for barcode-compliant sequences.
Table 2.
Distance (%K2P) with standard error, Barcode Index Number (BIN), and sequence composition summary for barcode-compliant sequences.