Fig 1.
Cell number distribution for all embryos on day 3.
Fig 2.
Division behaviors and cell number (n = 799).
(A) In <5C and 5–6C groups, FR was the main cause of blastomere loss when compared to other groups (P < 0.01). (B) Comparing to 7–8C embryos, DC became the leading abnormal behavior in 9–10C and >10C groups, P < 0.01 respectively. FR: fragmentation; DC: direct division; NB: normal behavior.
Fig 3.
Cell number, cleavage behavior and developmental potential.
(A) Cell number and developmental potential. Blastocyst formation rate and good quality blastocyst formation rate increased with cell number, and reached high levels in the 7–8C and >10 C groups. (B) Growth rate, division behaviors and embryo viability. DC decreased blastocyst formation rate in ≥5 C embryos significantly (P < 0.01). In embryos with normal division behavior, embryo development potential increased with cell number (P < 0.01). FR: fragmentation; DC: direct division; NB: normal behavior.
Fig 4.
Different morphokinetics parameters in low- and high cell number NB embryos.
(A) The first three mitosis cycles of NB embryos from insemination to 8 cells. cc1: duration of first cell cycle or mitosis, from completion of 2 cells to insemination; Ecc2: duration of second embryo cell cycle, from 2 to 4 cells; Ecc3: duration of third embryo cell cycle, from 4 to 8 cells. (B) Different morphokinetics parameters in low- and high cell number NB embryos. <5 C: NB embryos in <5 C embryos; 5− 8C: NB embryos in 5–8C embryos; > 8C: NB embryos in >8 C embryos. **P < 0.01.
Fig 5.
Different morphokinetics parameters in good quality and poor quality blastocysts.
Good: Good quality blastocysts; Poor: Poor quality blastocysts. **P < 0.01.
Fig 6.
Implantation rate and live birth rate in NB embryos with different cell number.