Table 1.
Species identification and geographic origin of the seventeen Gambierdiscus strains used in growth rate experiments.
Fig 1.
Growth rates of Gambierdiscus strains versus culture salinity of 10–60.
Each point is the mean of triplicate measurements, and the bars represent standard deviation (SD). Black arrows represent the maximum growth rate for a species.
Fig 2.
Average growth response of Gambierdiscus species to salinity of 10–60.
For ease of viewing, error bars shown in Fig 1 are omitted here.
Table 2.
Gambierdiscus strain numbers, growth rate, coefficient of variation among strains, and intraspecific differences for each species and salinity (10–60).
Fig 3.
Gambierdiscus growth rate responses (black squares) to salinities of 10–60 and simulated growth curves (red line) by polynomial regressive analysis.
(A) G. belizeanus (BP Mar10_6, BP Mar10_7, BP Mar10_22, FC Dec10_13), (B) G. caribaeus (BP Aug08, FC Nov09_4, SH Nov09_3), (C) G. carolinianus (SH Mar10_12, BB Apr10_3, BP May10_1), (D) G. carpenteri (KML1), (E) G. pacificus (3S0509-27, 3S0510-19), (F) G. silvae (FC May10_9), (G) Gambierdiscus sp. type 4 (1D0509-16, 1D0510-22), and (H) Gambierdiscus sp. type 5 (DS0511-03).
Table 3.
Gambierdiscus species growth parameters at salinities of 10–60.
Individual growth rate measurements were fitted to polynomial curves. The polynomial equations were used for growth parameter estimation: μm, maximum growth rate; Sm, salinity of maximum growth; Sopt, salinity of optimum growth range (μ≥0.8×μm); So, the lower salinity limit for growth; Su, the upper salinity limit for growth.
Fig 4.
Growth rates of Gambierdiscus strains versus culture irradiance of 55–400μmol photons · m-2 · s-1.
Each point is the mean of triplicate measurements, and the bars represent standard deviation (SD). Black arrows represent the maximum growth rate for a species.
Fig 5.
Average growth response of Gambierdiscus species to irradiance of 55–400μmol photons · m-2 · s-1.
For ease of viewing, error bars shown in Fig 4 are omitted here.
Table 4.
Gambierdiscus strain numbers, growth rate, coefficient of variation among strains, and intraspecific difference for each species and irradiance (55–400μmol · photons · m-2 · s-1).
Fig 6.
Growth rates of Gambierdiscus strains versus culture temperature of 16–36°C.
Each point is the mean of triplicate measurements, and the bars represent standard deviation (SD). Black arrows represent the maximum growth rate for a species.
Fig 7.
Average growth response of Gambierdiscus species to temperatures of 16–36°C.
For ease of viewing, error bars shown in Fig 6 are omitted here.
Table 5.
Gambierdiscus strain numbers, growth rate, coefficient of variation among strains, and intraspecific differences for each species and temperature (16–38°C).
Fig 8.
Gambierdiscus growth rate responses (black squares) at 16–36°C and simulated growth curves (red lines) by polynomial regressive analysis.
(A) G. belizeanus (BP Mar10_6, BP Mar10_7, BP Mar10_22, FC Dec10_13), (B) G. caribaeus (BP Aug08, FC Nov09_4, SH Nov09_3), (C) G. carolinianus (SH Mar10_12, BB Apr10_3, BP May10_1), (D) G. carpenteri (KML1), (E) G. pacificus (3S0509-27, 3S0510-19), (F) G. silvae (FC May10_9), (G) Gambierdiscus sp. type 4 (1D0509-16, 1D0510-22), and (H) Gambierdiscus sp. type 5 (DS0511-03).
Table 6.
Gambierdiscus species growth parameters in response to temperature (16–38°C).
Individual growth rate measurements were fitted to polynomial curves and equations. The polynomial equations were used for growth parameter estimation: μm, maximum growth rate; Tm, temperature of maximum growth; Topt, temperature of optimum growth range (μ≥0.8×μm); To, the lower temperature limit for growth; Tu, the upper temperature limit for growth.
Table 7.
Comparison of Gambierdiscus species growth rates between the current and previous studies.