Fig 1.
Longitudinal Rotarod performance measures of HIV-1 Tg and age-matched WT rats.
In the longitudinal measure, the transgene state (group) and age were found to be significant predictors of Rotarod performance [p<0.05], with the group and age interaction not statistically significant [p>0.05]. By week 8, control rats demonstrated significantly different Rotarod times compared to baseline, whereas Tg rats did not show a significant difference at any time point. Error bars reflect standard error of the mean (SEM) values. * reflect differences between WT and Tg rats (p<0.05).
Fig 2.
Longitudinal Open Field behavior comparison between HIV-1 Tg and age-matched WT rats.
Open field measures were collected and plotted to the nearest whole age number (weeks): (A) Total Exploratory Activity (B) Total distance (Internal Zone) (C) Total distance (External zone) (D) Number of Line Crossings (E) Rearing time and (F) Rearing Time (External zone). The transgene state (group) and age were found to be significant predictors of performance [p<0.05], with the group and age interaction not statistically significant [p>0.05] except for total distance (External Zone). (G) Depicts representative track plots for 16 week-old WT and Tg rats. Each track represents the total activity, line crossings and distance traveled in the internal zone by the subject during the 30 min time period of the test. The WT track crosses into the center portion of the maze at regular intervals and exhibits high exploratory activity while the Tg rat remains in close proximity to the walls of the maze and shows decreased exploratory activity. Error bars reflect standard error of the mean (SEM) values. * reflect differences between WT and Tg rats (p<0.05).
Table 1.
Open Field activity F statistics.
Fig 3.
18F-FDG uptake analysis between Tg and WT rats by PET scan (longitudinal study group).
Assessment of metabolic activity, determined by 18F-FDG uptake (SUV) normalized to whole brain uptake was performed for specific brain regions: (A) Striatum, (B) Cortex and (C) Hippocampus. Differences were not statistically significant at any time point (p>0.05; Student’s t-test). Error bars reflect standard deviation (SD) values.
Fig 4.
18F-FDG uptake analysis between Tg and WT rats by PET scan (cross-sectional study group).
Assessment of metabolic activity in (A) young (12 week-old) rats and (B) middle-aged (29 week-old) rats, determined by 18F-FDG uptake (SUV) normalized to whole brain uptake was performed for Striatum, Cortex and Hippocampus. Differences were not statistically significant between the Tg and WT rats at either age (p>0.05; unpaired Student’s t-tests). Error bars reflect standard deviation (SD) values.
Fig 5.
14C-DG uptake analysis between Tg and WT rats by autoradiography.
A cross-sectional analysis of 14C-DG (nCi/g) uptake was performed on 8 month-old HIV-1 Tg and aged-matched WT rats for specific brain regions: (A) Striatum, (B) Cortex and (C) Hippocampus. Differences of radioactivity concentrations (nCi/g) were not statistically significant (p>0.05; Mann-Whitney U-test). Error bars reflect standard deviation (SD) values.