Fig 1.
a) Photo of the arena set-up with one animal at the contact window (red circle). b) The contact window when closed and c) the open contact window. d) Schematic view of the arena with context regions as used for video scoring. The standard equipment for the experiments is colored in grey, the context regions that were used for video scoring are marked in different colors and inscribed with following abbreviations: CC = contact corners (dark blue); CR = contact region (light blue); CW = contact window (turquoise); F = food; FR = food region (green); H = red Plexiglas house; M = microphone, hanging from the top (see a); MB = male bedding (red); NR = neutral region (yellow); W = water.
Table 1.
Number of songs per recording night and context region.
Given is the number of songs per context region in each recording night and the number of songs in each recording night (sum), separately for each pair of females and summed over all experiments (sum all). The number of songs per night for the five repetitions is given in brackets. Context regions are: FR = food region, NR = nest region, CC = contact corners, CR = contact region.
Fig 2.
Frequency-distribution of the Center of Gravity of syllable frequency.
Data for night 1 (without direct contact) are shown in the upper panel, data for nights 2–4 (with direct contact) in the lower panel. The vertical line is drawn at 45 kHz and separates low-frequency and high-frequency syllables.
Fig 3.
Songs and syllables in the different recording nights.
a) The number of songs per night for each individual pair of females from sets A and B, as well as the mean number of songs per night in sets A and B. b) The mean number of songs in the different context regions in the four recording nights. c) The first two linear discriminant functions (LD) of syllable parameters in different recording nights. Ellipses show the 95% confidence interval around the group means. The contribution of the two linear discriminants is given below the axis. Arrows indicate the direction of the most influencing parameters on the separation of the data; for the loadings of these and the other syllable parameters see Table 3a.
Table 2.
Song parameters in different recording nights and context regions in night 1 and nights 2, 3, 4.
Shown is the mean ± sd and the p-value of pairwise comparisons (Wilcoxon signed rank and rank sum tests) for the different recording nights (a), and context regions in night 1 (b) and nights 2, 3, and 4 (c).
Table 3.
Syllable parameters in different recording nights and context regions in night 1 and nights 2, 3, 4.
Shown is the mean ± sd for each night or region, the loadings of the first 3 linear discriminant functions, and the p-value of pairwise comparisons (Wilcoxon rank sum tests) for the different recording nights (a), and context regions in night 1 (b) and nights 2, 3, and 4 (c).
Fig 4.
Syllables in different context regions.
a) The first two linear discriminant functions (LD) of syllable parameters in different context regions in nights without direct contact (night 1). b) The first two linear discriminant functions (LD) of syllable parameters in different context regions in nights with direct contact (nights 2, 3, 4). Ellipses in the scatterplots show the 95% confidence interval around the group means. The contribution of the two linear discriminants is given below the axis. Arrows indicate the direction of the most influencing parameters on the separation of the data; for the loadings of these and the other syllable parameters see Table 3b and 3c.
Fig 5.
Songs and syllables in different encounters at the contact window.
a) The amount of songs recorded during different encounters at the contact window (solitary situations, face to face and non-face to face encounters), and in the contact corners in percent, shown separately for nights 2, 3, and 4. Given is the mean ± the 95% confidence interval for each group. b) The mean (± 95% confidence interval) of the Center of Gravity in syllables from solitary situations, face to face and non-face to face encounters in the contact region, and contact corner syllables. c) The mean (± 95% confidence interval) of the frequency slope in syllables from solitary situations, face to face and non-face to face encounters, and contact corner syllables. Abbreviations for the different types of encounters are: solitary situations = solitary; face to face encounters = face; non-face to face encounters = non-face. The test statistics for pairwise comparisons can be found in Table 4.
Table 4.
Song and syllable parameters in different encounters at the contact window.
Shown is the mean ± sd for each type of encounter, the loadings of the first 2 linear discriminant functions, and the p-value of pairwise comparisons (Wilcoxon signed rank and rank sum tests). Abbreviations for pairwise comparisons are: sol = solitary, nf = non-face to face, ftf = face to face.