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Fig 1.

CK19 and GPC3 expression in different histological variation of HCC.

Thin trabecular HCC was usually accompanied by the pseudoglandular structure (blue arrow head). This histological variant mostly showed CK19−/GPC3− expression (A). Majority of SHCCs showed CK19+/GPC3+ expression. But nearly half of CK19+/GPC3+ cases with thick trabecular structure also showed abundant fibrous stroma. According to the parenchymal cell to fibrous stroma ratio, they do not fully meet the diagnostic criteria of SHCC (B, C). Most of the compact form of HCC present with CK19−/GPC3+ expression. CD34 staining showed that sinusoid-like blood spaces were not abundant compared to other histological variants (D).

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Fig 1 Expand

Table 1.

The relationship between the histological variation and immune-subtypes of HCC.

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Table 1 Expand

Table 2.

The correlation between immune-phenotype and histological grading of HCC.

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Table 2 Expand

Table 3.

Correlation between the immune-phenotype and clinical staging of HCC.

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Table 4.

The correlation in TNM staging between any two immune- subtype HCC.

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Table 5.

Univariate analysis with respect to tumor recurrent.

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Fig 2.

The log-rank test showed a significant difference among the survival curves of three immunosubtypes of HCC (log-rank statistic = 22.61, d.f. = 2, P < 0.01).

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Fig 2 Expand

Table 6.

Multivariable Cox proportional hazards regression analysis.

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Table 6 Expand