Fig 1.
Symptoms for P. capsici induced root rot of pepper.
A, The phenotype of CM334 9 days post-inoculation. B, The phenotype of NMCA10399 9 days post-inoculation. C, Roots of CM334 9 days post-inoculation. D, Roots of NMCA10399 9 days post-inoculation. The arrows indicate the stem at the soil line.
Table 1.
Summary of sequencing data for parental lines and DNA pools.
Table 2.
Distribution of SLAFs, polymorphic SLAFs and high-quality polymorphic SLAFs over the pepper chromosomes.
Fig 2.
Graph of Δ(SNP-index) from analysis of SLAF-seq marker-trait association.
The X-axis represents chromosomal position and the Y-axis represents Δ(SNP-index). The blue dashed line indicates the association-threshold. A region related to PRR resistance was identified in pepper chromosome 10 (217.17–233.56 Mb interval) where the Δ(SNP-index) consistently exceeded the association-threshold. Higher Δ(SNP-index) indicates stronger association.
Fig 3.
Gel images of 10 polymorphic SSR markers in parents and pools.
P1, P2, PR and PS represent the resistant parent CM334, susceptible parent NMCA10399, resistant pool and susceptible pool, respectively.
Fig 4.
Genetic linkage map of PhR10 constructed from the BC1 population of (CM334×NMCA10399)×NMCA10399.
Map distance in Kosambi centiMorgans (cM) is on the left, SSR markers are arranged on the right.