Fig 1.
The schematic representation of different events staring from library generation by 454 sequencing, assembly, annotation and other analysis performed.
Fig 2.
Distribution of selected GO terms based on NCBI-BLAST analysis of mulberry transcriptome generated from drought stressed leaf tissue.
Table 1.
Annotated PUFs using the computational tools.
Fig 3.
a) Option to input of contigs identified from a genome by using next-generation sequencing, b) Output of Pfam and CDD, c) Gene identification and its elements, d) Amino acid sequence of a given contig.
Table 2.
Sequence and structure based annotation of selected PUFs.
Fig 4.
Phylogenetic analysis of selected PUF.
a) Multiple sequence alignment of mulberry PUF39 with homologous genes from other plant genomes. b) Unrooted phylogenetic tree of PUF39. The Genbank accession numbers of the sequences are Ricinus communis (XP_002510750.1), Populus trichocarpa (XP_002301890.1), Malus domestica (XP_008381650.1), Theobroma cacao (XP_007018633.1), Brassica rapa (XP_009149581.1), Arabidopsis lyrata subsp. Lyrata (XP_002890421.1), Arabidopsis thaliana (NP_001117322.1), Solanum tuberosum (XP_006352862.1), Phaseolus vulgaris (XP_007131503.1) and Glycine max (XP_003540084.1).
Fig 5.
Expression analysis of selected PUFs by qRT-PCR in leaf tissue of mulberry genotype, Dudia white.
Relative transcript levels of MaUSP-like (a), MaPLATZ1-like (b), and MaRRM1-like (c) genes under salinity and oxidative stresses. Total RNA was isolated from the leaf tissues of mulberry twigs exposed to 250mM NaCl (salinity stress) and 15μM methyl viologen (oxidative stress) at 6, 12, 24, and 48 hours after stress imposition. Transcripts were normalized to the expression of the elongation factor gene. The data shown are mean ± SE from three independent experiments. Asterisk indicates the significant difference between control and treatments at p = 0.05.
Table 3.
Tools used in the study.