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Table 1.

Geographic origins of Colletotrichum gloeosporioides populations, the number of isolates represented in each population and their hosts.

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Table 2.

Characteristics of 23 polymorphic microsatellite markers developed in this study for population genetic diversity analysis of Colletotrichum gloeosporioides isolates.

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Table 2 Expand

Table 3.

Diversity indices of the microsatellite loci used in the study.

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Table 3 Expand

Table 4.

Summary of the population diversity indices averaged over 23 loci.

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Table 5.

Analysis of molecular variance among and within populations, and within individuals of Colletotrichum gloeosporioides populations based on 23 SSR loci.

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Table 6.

Pairwise genetic distance based on FST matrix, a measure of divergence among the Colletotrichum species populations.

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Fig 1.

Unweighted Neighbor-joining tree using the simple matching similarity coefficient based on 23 microsatellite markers for the 163 isolates of Colletotrichum gloeosporioides isolated from citrus in Ethiopia.

The tree shows the clustering pattern of isolates from the four C. gloeosporioides populations. The populations are color coded as follows: Central region (red), Northwest region (green), South region (blue) and Southwest region (violet).

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Fig 2.

Principal coordinates analysis (PCoA) bi-plot showing the clustering of the 163Colletotrichumgloeosporioides isolates based on 23 microsatellite loci.

The four populations are color coded as follows: Central region (red), Northwest region (green), South region (blue) and Southwest region (yellow). Percentages of variation explained by the first 3 axes (1, 2, and 3) are 36.16%, 11.6% and 8.68%, respectively.

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Fig 3.

Bayesian model-based estimation of population structure (K = 3) for the 163 Colletotrichum gloeosporioides isolates in four pre-determined populations (x-axis): Central Ethiopia (CE), Northwest Ethiopia (NEW), South Ethiopia (SE) and Southwest Ethiopia (SWE).

Each group is separated by a black vertical line. Numbers in the y-axis show coefficient of membership/assignment.

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