Fig 1.
grp3-1 loss-of-function mutant analysis.
a Relative expression of AtGRP3 transcripts analyzed through real-time quantitative PCR of Col and grp3-1 mutant. b Summarized data for root length measurements of 2-week-old plants. Error bars indicate standard error. *** indicates p≤ 0.005 and **** indicates p≤ 0.001.
Fig 2.
Relative expression of cell elongation and/or division molecular markers in Col and grp3-1.
Quantitative real time PCR for a COB (At5g60920). b KOR1 (At5g49720). c CESA6 (At5g64740). d POM1 (At1g05850). e DWF1 (At3g19820). f BRI1 (At4g39400). g CDC48 (At3g09840). h CYCB1;2 (At5g06150). Error bars indicate standard error. * indicates p≤ 0.05, *** indicates p≤ 0.005 and **** indicates p≤ 0.001.
Fig 3.
Confocal analysis of root cells.
a-c Division pattern of stele cells. * labels stele rows. a Col wild-type. b grp3-1 individual presenting normal division pattern. c grp3-1 individual presenting abnormal division pattern. d Counting of number of stele cell rows. e Root diameter measurements. f Cell length measurements. Error bars indicate standard error. * indicates p≤ 0.05, ** indicates p≤ 0.01, *** indicates p≤ 0.005 and **** indicates p≤ 0.001.
Fig 4.
Analyses regarding implication of AtGRP3 in Al signaling in Col wild-type and grp3-1 knockout plants.
a Reduction in root growth resulting from Al exposure. Error bars indicate standard error. b Relative expression of AtWAK1 transcripts analyzed through real-time quantitative PCR of Col and grp3-1 mutant. Error bars indicate standard error. *** indicates p≤ 0.005 and **** indicates p≤ 0.001. c Quantitative real time PCR for AtGRP3 and AtWAK1 in Col plants submitted to Al for 0h and 8h. Error bars indicate standard error. * indicates p≤ 0.05 and **** indicates p≤ 0.001.