Fig 1.
Acclimation of CTmin in captive Carlia longipes.
Critical thermal minimum temperatures [°C± 2SE] of captive individuals of Carlia longipes acclimated to a temperature regime of 18–22°C for different time periods measured in weeks. Every week, 3 to 4 previously untested individuals were added to the experimental group and other individuals retested. Past 8 weeks acclimation, only previously tested individuals were used. Predictor line for an average sized animal (4.88g) is shown (dashed). Values approach an asymptote of 7.13°C for an average sized animal, with 95% (7.36°C) of this acclimation completed after 17 weeks (dotted line).
Fig 2.
Acclimation of CTmin in wild Carlia longipes.
Critical thermal minimum temperatures [°C ± 2SE] of wild individuals (3 to 8 individuals per group) of Carlia longipes collected in different months of a year. No statistically significant differences were observed (ANOVA: F(5, 25) = 1.05, p = 0.4). Lines show mean CTmin of wild animals (9.67°C; dashed) and predicted cold acclimated CTmin of an average-sized animal of 4.88 g body mass (7.13°C; dotted).
Fig 3.
Minimum habitat temperatures of Carlia longipes.
Density distribution of habitat temperatures (histograms) and minimum temperatures recorded by the Bureau of Meteorology (dashed lines) at different times of year. Habitat temperatures represent records collected at 5 am every day (coldest time of day) over a two-week period in eight different microhabitats representative of the locations that Carlia longipes is commonly observed in. Vertical dotted lines show acclimation regime used in this study.
Fig 4.
Acclimation of CTmin in 22 reptile species.
Critical thermal minimum temperatures [°C ± 2SE] of 22 warm acclimated (0 days acclimation) and partially acclimated (2 to 4 weeks acclimation) reptile species. Solid line shows predictions by linear mixed effects model with lowest AIC. Values approach an asymptote of 2.61°C. 95% of acclimation is completed at 7.3 weeks (dotted black reference line). Predictions by other models of inferior fit (dotted black reference line) and acclimation response of Carlia longipes (dashed black line) are shown for comparison. The model of best fit was described by the following equation: (2)