Fig 1.
Functional categorization of up-regulated and down-regulated proteins in jejunal upper villus epithelial cells of weaning piglets.
The differentially expressed proteins were clustered using Cluster 3.0 with k-means clustering. The up-regulated (Up) and down-regulated (Down) proteins enriched groups were selected (indicated by arrowheads). Cellular component, molecular function, and biological process ontology of the Up proteins (arrowheads pointing up) and Down proteins (arrowheads pointing down) were performed using WEGO program. The GO terms with a P-value ≤ 0.05 (Pearson Chi-Square test between the protein numbers of Up and Down proteins) were selected.
Fig 2.
Proteins enriched in Golgi vesicle transport in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in Golgi vesicle transport between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in Golgi vesicle transport were selected and clustered by Cluster 3.0.
Fig 3.
Proteins enriched in protein amino acid glycosylation in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in protein amino acid glycosylation between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in protein amino acid glycosylation were selected and clustered by Cluster 3.0.
Fig 4.
Proteins enriched in secretion by cell in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in secretion by cell between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in secretion by cell were selected and clustered by Cluster 3.0.
Fig 5.
Proteins enriched in transmembrane transport in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in transmembrane transport between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in transmembrane transport were selected and clustered by Cluster 3.0.
Fig 6.
Proteins enriched in ion transport in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in ion transport between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in ion transport were selected and clustered by Cluster 3.0.
Fig 7.
Proteins enriched in nucleotide catabolic process in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in nucleotide catabolic process between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in nucleotide catabolic process were selected and clustered by Cluster 3.0.
Fig 8.
Proteins enriched in translational initiation in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in translational initiation between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in translational initiation were selected and clustered by Cluster 3.0.
Table 1.
Effects of weaning on the expression of proteins in mammalian target of rapamycin signaling pathway (mTOR) in jejunal upper villus epithelial cells of piglets.
Table 2.
Effect of weaning on the expression of I-FABP, Bcl-2, and caspase-3 in jejunal upper villus epithelial cells of piglets.
Fig 9.
Proteins enriched in energy derivation by oxidation of organic compounds in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in energy derivation by oxidation of organic compounds between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in energy derivation by oxidation of organic compounds were selected and clustered by Cluster 3.0.
Fig 10.
Proteins enriched in lipid catabolic process in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in lipid catabolic process between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in lipid catabolic process were selected and clustered by Cluster 3.0.
Fig 11.
Proteins enriched in monosaccharide catabolic process in jejunal upper villus epithelial cells of weaning piglets.
There was a remarkable relationship (P< 0.05) in monosaccharide catabolic process between Up and Down proteins based on WEGO program analysis. The Up and Down proteins enriched in monosaccharide catabolic process were selected and clustered by Cluster 3.0.
Fig 12.
The enrichment of differentially expressed proteins in glycolysis pathway in jejunal upper villus epithelial cells of weaning piglets.
A protein with ≥ 1.2-fold or ≤ 0.8-fold difference between W1d, W3d, W5d or W7d and W0d and a P-value ≤ 0.05 was regarded as being differentially expressed. The glycolysis pathway enrichment was performed using KEGG database.
Fig 13.
The enrichment of differentially expressed proteins in citrate cycle pathway in jejunal upper villus epithelial cells of weaning piglets.
A protein with ≥ 1.2-fold or ≤ 0.8-fold difference between W1d, W3d, W5d or W7d and W0d and a P-value ≤ 0.05 was regarded as being differentially expressed. The citrate cycle pathway enrichment was performed using KEGG database.
Table 3.
The mRNA expression of genes related to glycolysis, fatty acids catabolism, and citrate cycle in jejunal upper villus epithelial cells of weaning piglets.