Table 1.
Viruses used in the standardisation of Margarita strain real-time TaqMan assay.
Fig 1.
Experimental procedures to examine superinfection exclusion in PBMCs and ST cells.
Fig 2.
The animals persistently infected with Cat01 were clinically protected after infection with the virulent Margarita strain.
Means and standard deviations of the daily individual clinical score values after CSFV virulent Margarita strain infection are represented. Dark grey bars indicate the standard deviation values for group A. The clinical score values are defined in the Materials and Methods section.
Fig 3.
Swine persistently infected with the CSFV Cat01 strain were protected from the typical viraemia generated by the CSFV Margarita strain.
(A) Daily detection of CSFV RNA through generic qRT-PCR in sera [39]. The Ct values from group A (CSFV acutely Margarita-infected wild boars; 1–3) and group B (CSFV-superinfected wild boars; 4–6) are represented in black and grey colours, respectively. (B) Daily detection of CSFV RNA Margarita strain through specific qRT-PCR in serum. The Ct mean values from group A (CSFV acutely infected wild boars; 1–3) and group B (CSFV-superinfected wild boars; 4–6) are represented in black and grey colours, respectively. Positive results for the CSFV RNA detection [39] were considered for Ct values equal to or less than 42, indicated with a dashed line. Positive results for the specific CSFV RNA Margarita strain detection were considered for Ct values equal to or less than 38, indicated with a dotted line
Table 2.
Swine persistently infected with the CSFV Catalonia strain are protected from CSFV Margarita strain infection in tissue samples.
Table 3.
CSFV interference in the PBMCs from superinfected hosts.
Fig 4.
Sequence analysis of the partial E2 sequence does not detect the CSFV Margarita RNA in the tissues from superinfected animals.
The Margarita and Cat01 viral strain sequences used as viral inocula in the animal infection experiments were considered as references. Sequences from sera, tonsil and spleen samples from group A (CSFV acutely infected wild boars; 1, 3) and group B (CSF-superinfected wild boars; 4, 5) are shown. Differences in the nucleotide sequences between the CSFV Margarita and Catalonia 01 strains are shown in grey and dark grey, respectively.