Fig 1.
Time-course fresh water loss of Pyrus betulaefolia seedling during dehydration for 0, 1, 3 and 6 h in an ambient environment at 26°C, followed by recovery in water at 26°C for 24 h.
(A-E). Electrolyte leakage and relative water content (RWC) analysis of Pyrus betulaefolia leaves. (F). Content of photosynthetic rate (Pn) (G); content of stomatal conductance (Gs) (H); content of transpiration rate (Tr) (I).
Table 1.
Summary of read numbers based on the RNA-Seq data from Pyrus betulaefolia pear during dehydration.
Fig 2.
Differentially expressed genes between different libraries.
Up-regulated (red) and down-regulated (green) genes were quantified (A). The ten comparison results are shown. Venn diagram showing the differentially expressed genes at each of the five time points associated with drought stress. (B) Up-regulated genes. (C) Down-regulated genes.
Table 2.
Over-represented GO terms for specific highly expressed genes at a given time point compared to the other time points.
Fig 3.
GO categories for the genes identified. Y-axis (left) represents the percentage of genes identified in this study; Y-axis (right) represents the actual gene number.
The genes were annotated into three main categories: biological process, cellular component and molecular function (X-axis).
Fig 4.
Clustering and gene ontology enrichment of regulated genes.
Genes displaying some degree of regulation were clustered using Hierarchical Clustering. A description of the expression pattern and the number of transcripts belonging to the cluster form the title of each chart. Expression values were normalized and scaled to between –1.0 and 1.0 (Y-axis). Enriched “Biological Process” GO terms, generated in BinGO are listed at the bottom of each cluster. For Clusters 12, 13, 14, 16 and 18, no over-represented “Biological Process” terms were detected. The number of genes associated with an over-represented GO term in the cluster and the number of genes associated with the same GO term in the Pyrus transcriptome are shown in parentheses. Specific genes belonging to each cluster can be found in S3 File. 2 (D1), 3 (D3), 4, (D6), 5 (DH24).
Fig 5.
Expression patterns of genes involved in ABA and GA metabolism.
Fig 6.
Quantitative RT-PCR validation of differential gene expression during dehydration.
The left Y-axis indicates the gene expression levels calculated by the RPKM method. The right Y-axis indicates the relative gene expression levels.