Fig 1.
qPCR quantification of 16S rRNA gene in the surface sediment samples of CRB (A) and HOP (B).
Values (log transformed) are expressed for the copy number per gram of sediment dry weight normalized to the DNA extraction yield. The box plot represents the first and third quartile, the blue dots on maximum/minimum values and the red cross indicate mean.
Fig 2.
Gene copy number of selected bacterial species (E. coli, ENT, and Pseudomonas spp.) normalized to 16S rRNA gene copy numbers in sediment samples collected for 4 months (June to September) at selected HOP.
The box plot represents the first and third quartile, the blue dots on maximum/minimum values and the red cross indicate mean.
Fig 3.
Gene copy number of (A) selected bacterial species (E. coli, ENT, and Pseudomonas spp.) and (B) selected antibiotic resistance genes (aadA, blaTEM, blaCTX‑M, blaSHV, and blaNDM) normalized to 16S rRNA gene copy numbers in the sediment samples collected in September 2013 from CRB, Tiruchirappalli, Tamil Nadu, India.
Fig 4.
Gene copy numbers of antibiotic resistance genes (blaTEM, blaCTX‑M, blaSHV, blaNDM and aadA,) normalized to 16S rRNA gene copy numbers in sediment samples collected for 4 months (June to September) at selected HOP.
The box plot represents the first and third quartile, the blue dots on maximum/minimum values and the red cross indicate mean.
Fig 5.
Principle Component Analysis (PCA), on correlation biplot (left panel) indicating the correlations among studied variables and biplot scores on axis 1 and 2 are representing 64.21%.
Agglomerative hierarchical clustering (AHC) computation based on Ward's method (right panel).